@inproceedings{8857ea6ad52541ba823d5b7ede9f1281,
title = "An integrated microfluidic system for antibiotic resistance gene identification capable differentiating live and dead of vancomycin-resistant enterococcus",
abstract = "This study presents an integrated microfluidic system which can identify the vancomycin resistant gene (vanA) from live hetero-bacteria automatically. In this study, a new approach to diagnose the vanA gene from live hetero-bacteria by using ethidium monoazide (EMA) and loop-mediated isothermal amplification (LAMP) was proposed and its feasibility was tested and verified. In addition, an integrated microfluidic system including a microfluidic chip and a home-made control system was also demonstrated. The experimental results showed that the developed system can detect the vanA gene from 'live' Enterococcus but not 'dead' Enterococcus successfully with a detection limit of 10 colony formation units (CFU). The entire process including the sample pre-treatment process could be automated within 1 hour. This is the first time that an integrated microfluidic system was demonstrated to diagnose the vanA gene from live bacteria by using a molecular diagnosis approach. With its high sensitivity and accuracy, the proposed system may be promising to verify antibiotic resistance genes from live hetero-bacteria which cannot be achieved by using the existing diagnostic methods.",
keywords = "live hetero-bacteria, loop-mediated isothermal amplification, microfluidic, vancomycin resistant gene",
author = "Chang, {Wen Hsin} and Yang, {Sung Yi} and Lin, {Yi Cheng} and Wang, {Chih Hung} and You, {Huey Ling} and Wu, {Jiunn Jong} and Lee, {Mel S.} and Lee, {Gwo Bin}",
note = "Publisher Copyright: {\textcopyright} 2016 IEEE.; 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 ; Conference date: 17-04-2016 Through 20-04-2016",
year = "2016",
month = nov,
day = "28",
doi = "10.1109/NEMS.2016.7758321",
language = "English",
series = "2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "591--594",
booktitle = "2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016",
address = "United States",
}