An immunogold single extracellular vesicular RNA and protein (AuSERP) biochip to predict responses to immunotherapy in non-small cell lung cancer patients

Luong T.H. Nguyen, Jingjing Zhang, Xilal Y. Rima, Xinyu Wang, Kwang Joo Kwak, Tamio Okimoto, Joseph Amann, Min Jin Yoon, Takehito Shukuya, Chi Ling Chiang, Nicole Walters, Yifan Ma, Donald Belcher, Hong Li, Andre F. Palmer, David P. Carbone, L. James Lee, Eduardo Reátegui*

*此作品的通信作者

研究成果: Article同行評審

19 引文 斯高帕斯(Scopus)

摘要

Conventional PD-L1 immunohistochemical tissue biopsies only predict 20%–40% of non-small cell lung cancer (NSCLC) patients that will respond positively to anti-PD-1/PD-L1 immunotherapy. Herein, we present an immunogold biochip to quantify single extracellular vesicular RNA and protein (AuSERP) as a non-invasive alternative. With only 20 μl of purified serum, PD-1/PD-L1 proteins on the surface of extracellular vesicles (EVs) and EV PD-1/PD-L1 messenger RNA (mRNA) cargo were detected at a single-vesicle resolution and exceeded the sensitivities of their bulk-analysis conventional counterparts, ELISA and qRT-PCR, by 1000 times. By testing a cohort of 27 non-responding and 27 responding NSCLC patients, AuSERP indicated that the single-EV mRNA biomarkers surpass the single-EV protein biomarkers in predicting patient responses to immunotherapy. Dual single-EV PD-1/PD-L1 mRNA detection differentiated responders from non-responders with an accuracy of 72.2% and achieved an NSCLC diagnosis accuracy of 93.2%, suggesting the potential for AuSERP to provide enhanced immunotherapy predictions and cancer diagnoses within the clinical setting.

原文English
文章編號e12258
期刊Journal of Extracellular Vesicles
11
發行號9
DOIs
出版狀態Published - 9月 2022

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