TY - GEN
T1 - AC electrokinetics assisted impedance biosensors for rapid bacteria detection
AU - Ho, Yi Tse
AU - Pan, Chun Liang
AU - Sheu, Jeng-Tzong
AU - Wang, Yu Kuo
AU - Wu, Tung-Kung
PY - 2012/12/1
Y1 - 2012/12/1
N2 - Early detection of living bacteria attracts a lot of attention in infection diseases applications, especially at low concentration. Nowadays, the detection of specific bacteria faces challenges including enhancement of trapping efficiency, and increase of sensor sensitivity and specificity. In this study, we improved the trapping efficiency and increased sensor sensitivity and specificity by using AC electrokinetics and selective modification of antibody on gold electrode in a new structure of gold electrode design. COMSOL simulations of AC electrokinetics include dielectrophoresis (DEP), and AC electroosmosis (ACEO) were performed on different electrode designs and bias configurations. The simulation results were consistent with the fluorescence observations showing that the bacteria were trapping selectively on gold electrodes. The impedance measurements for Vibrio parahaemolyticus (Vp) detection at 105 cfu/ml with AC elctrokinetics showed a nearly 90% change in normalized impedance change (NIC).
AB - Early detection of living bacteria attracts a lot of attention in infection diseases applications, especially at low concentration. Nowadays, the detection of specific bacteria faces challenges including enhancement of trapping efficiency, and increase of sensor sensitivity and specificity. In this study, we improved the trapping efficiency and increased sensor sensitivity and specificity by using AC electrokinetics and selective modification of antibody on gold electrode in a new structure of gold electrode design. COMSOL simulations of AC electrokinetics include dielectrophoresis (DEP), and AC electroosmosis (ACEO) were performed on different electrode designs and bias configurations. The simulation results were consistent with the fluorescence observations showing that the bacteria were trapping selectively on gold electrodes. The impedance measurements for Vibrio parahaemolyticus (Vp) detection at 105 cfu/ml with AC elctrokinetics showed a nearly 90% change in normalized impedance change (NIC).
UR - http://www.scopus.com/inward/record.url?scp=84874173327&partnerID=8YFLogxK
U2 - 10.1109/BioCAS.2012.6418513
DO - 10.1109/BioCAS.2012.6418513
M3 - Conference contribution
AN - SCOPUS:84874173327
SN - 9781467322935
T3 - 2012 IEEE Biomedical Circuits and Systems Conference: Intelligent Biomedical Electronics and Systems for Better Life and Better Environment, BioCAS 2012 - Conference Publications
SP - 49
EP - 52
BT - 2012 IEEE Biomedical Circuits and Systems Conference
T2 - 2012 IEEE Biomedical Circuits and Systems Conference: Intelligent Biomedical Electronics and Systems for Better Life and Better Environment, BioCAS 2012
Y2 - 28 November 2012 through 30 November 2012
ER -