A sensitive and versatile in situ hybridization protocol for gene expression analysis in developing amniote brains

Pei Shan Hou, Takuma Kumamoto, Carina Hanashima*

*此作品的通信作者

研究成果: Chapter同行評審

1 引文 斯高帕斯(Scopus)

摘要

The detection of specific RNA molecules in embryonic tissues has wide research applications including studying gene expression dynamics in brain development and evolution. Recent advances in sequencing technologies have introduced new animal models to explore the molecular principles underlying the assembly and diversification of brain circuits between different amniote species. Here, we provide a step-by-step protocol for a versatile in situ hybridization method that is immediately applicable to a range of amniote embryos including zebra finch and Madagascar ground gecko, two new model organisms that have rapidly emerged for comparative brain studies over recent years. The sensitive detection of transcripts from low to high abundance expression range using the same platform enables direct comparison of gene of interest among different amniotes, providing high-resolution spatiotemporal information of gene expression to dissect the molecular principles underlying brain evolution.

原文English
主出版物標題Methods in Molecular Biology
發行者Humana Press Inc.
頁面319-334
頁數16
DOIs
出版狀態Published - 2017

出版系列

名字Methods in Molecular Biology
1650
ISSN(列印)1064-3745

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