TY - GEN
T1 - A new pathogen detection system by utlizing nanogold modified specific probe and vancomycin coated magnetic beads on an integrated microfluidic device
AU - Wang, Chih Hung
AU - Chang, Chia Jung
AU - Wu, Jiunn Jong
AU - Lee, Gwo Bin
PY - 2013
Y1 - 2013
N2 - A simple, novel assay for pathogen diagnosis was implemented on a new integrated microfluidic system using a nanogold modified DNA probe and vancomycin conjugated magnetic beads. The developed microfluidic device was fabricated by microfluidic technology and composed of many micro-modules. The pneumatically-driven, suction-type micropump, microvalves, microchannels and loading chambers were integrated on a single chip to automate the entire diagnosis process. The bacteria were captured by vancomycin conjugated magnetic beads and hybridized with specific nanogold probe. The diagnostic results were determined simply with color change of nanogold by visually bare-eye observation and may be detected optically if necessary. The whole analytic processes only need 25 min from sample and reagent added to results obtained. The developed platform may provide a promising tool for fast diagnosis of bacterial infections.
AB - A simple, novel assay for pathogen diagnosis was implemented on a new integrated microfluidic system using a nanogold modified DNA probe and vancomycin conjugated magnetic beads. The developed microfluidic device was fabricated by microfluidic technology and composed of many micro-modules. The pneumatically-driven, suction-type micropump, microvalves, microchannels and loading chambers were integrated on a single chip to automate the entire diagnosis process. The bacteria were captured by vancomycin conjugated magnetic beads and hybridized with specific nanogold probe. The diagnostic results were determined simply with color change of nanogold by visually bare-eye observation and may be detected optically if necessary. The whole analytic processes only need 25 min from sample and reagent added to results obtained. The developed platform may provide a promising tool for fast diagnosis of bacterial infections.
UR - http://www.scopus.com/inward/record.url?scp=84875462603&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2013.6474185
DO - 10.1109/MEMSYS.2013.6474185
M3 - Conference contribution
AN - SCOPUS:84875462603
SN - 9781467356558
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 94
EP - 97
BT - IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
T2 - IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Y2 - 20 January 2013 through 24 January 2013
ER -