A near-infrared fluorescent probe with a substantial Stokes shift designed for the detection and imaging of β-galactosidase within living cells and animals

Yuan Pin Lo; Narayanasamy Nivetha; Sivan Velmathi; Shu Pao Wu

doi:10.1016/j.ymeth.2023.12.004

A near-infrared fluorescent probe with a substantial Stokes shift designed for the detection and imaging of β-galactosidase within living cells and animals

Yuan Pin Lo, Narayanasamy Nivetha, Sivan Velmathi, Shu Pao Wu^*

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摘要

β-Galactosidase serves as a pivotal biomarker for both cancer and cellular aging. The advancement of fluorescent sensors for tracking β-galactosidase activity is imperative in the realm of cancer diagnosis. We have designed a near-infrared fluorescent probe (PTA-gal) for the detection of β-galactosidase in living systems with large Stokes shifts. PTA-gal exhibits remarkable sensitivity and selectivity in detecting β-galactosidase, producing near-infrared fluorescent signals with a remarkably low detection limit (2.2 × 10^-5 U/mL) and a high quantum yield (0.30). Moreover, PTA-gal demonstrates biocompatibility and can effectively detect β-galactosidase in cancer cells as well as within living animals.

原文	English
頁（從 - 到）	10-18
頁數	9
期刊	Methods
卷	222
DOIs	https://doi.org/10.1016/j.ymeth.2023.12.004
出版狀態	Published - 2月 2024

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title = "A near-infrared fluorescent probe with a substantial Stokes shift designed for the detection and imaging of β-galactosidase within living cells and animals",

abstract = "β-Galactosidase serves as a pivotal biomarker for both cancer and cellular aging. The advancement of fluorescent sensors for tracking β-galactosidase activity is imperative in the realm of cancer diagnosis. We have designed a near-infrared fluorescent probe (PTA-gal) for the detection of β-galactosidase in living systems with large Stokes shifts. PTA-gal exhibits remarkable sensitivity and selectivity in detecting β-galactosidase, producing near-infrared fluorescent signals with a remarkably low detection limit (2.2 × 10-5 U/mL) and a high quantum yield (0.30). Moreover, PTA-gal demonstrates biocompatibility and can effectively detect β-galactosidase in cancer cells as well as within living animals.",

keywords = "Fluorescent probe, Near-infrared, Ovarian cancer cells, Stokes shift, β-Galactosidase",

author = "Lo, {Yuan Pin} and Narayanasamy Nivetha and Sivan Velmathi and Wu, {Shu Pao}",

note = "Publisher Copyright: {\textcopyright} 2023 Elsevier Inc.",

year = "2024",

month = feb,

doi = "10.1016/j.ymeth.2023.12.004",

language = "English",

volume = "222",

pages = "10--18",

journal = "Methods",

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T1 - A near-infrared fluorescent probe with a substantial Stokes shift designed for the detection and imaging of β-galactosidase within living cells and animals

AU - Lo, Yuan Pin

AU - Nivetha, Narayanasamy

AU - Velmathi, Sivan

AU - Wu, Shu Pao

PY - 2024/2

Y1 - 2024/2

N2 - β-Galactosidase serves as a pivotal biomarker for both cancer and cellular aging. The advancement of fluorescent sensors for tracking β-galactosidase activity is imperative in the realm of cancer diagnosis. We have designed a near-infrared fluorescent probe (PTA-gal) for the detection of β-galactosidase in living systems with large Stokes shifts. PTA-gal exhibits remarkable sensitivity and selectivity in detecting β-galactosidase, producing near-infrared fluorescent signals with a remarkably low detection limit (2.2 × 10-5 U/mL) and a high quantum yield (0.30). Moreover, PTA-gal demonstrates biocompatibility and can effectively detect β-galactosidase in cancer cells as well as within living animals.

AB - β-Galactosidase serves as a pivotal biomarker for both cancer and cellular aging. The advancement of fluorescent sensors for tracking β-galactosidase activity is imperative in the realm of cancer diagnosis. We have designed a near-infrared fluorescent probe (PTA-gal) for the detection of β-galactosidase in living systems with large Stokes shifts. PTA-gal exhibits remarkable sensitivity and selectivity in detecting β-galactosidase, producing near-infrared fluorescent signals with a remarkably low detection limit (2.2 × 10-5 U/mL) and a high quantum yield (0.30). Moreover, PTA-gal demonstrates biocompatibility and can effectively detect β-galactosidase in cancer cells as well as within living animals.

KW - Fluorescent probe

KW - Near-infrared

KW - Ovarian cancer cells

KW - Stokes shift

KW - β-Galactosidase

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U2 - 10.1016/j.ymeth.2023.12.004

DO - 10.1016/j.ymeth.2023.12.004

M3 - Article

C2 - 38154527

AN - SCOPUS:85181679446

SN - 1046-2023

VL - 222

SP - 10

EP - 18

JO - Methods

JF - Methods

ER -

A near-infrared fluorescent probe with a substantial Stokes shift designed for the detection and imaging of β-galactosidase within living cells and animals

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