TY - JOUR
T1 - A Feasibility Study of Broadband White Light Emitting Diode (WLED) Based Full-Field Optical Coherence Microscopy (FF-OCM) Using Derivative-Based Algorithm
AU - Anna, Tulsi
AU - Chang, Ting Wei
AU - Lai, Chih Ming
AU - Chiou, Arthur
AU - Kuo, Wen Chuan
N1 - Publisher Copyright:
© 2009-2012 IEEE.
PY - 2017/4
Y1 - 2017/4
N2 - Developing cost-effective and fast detection methods in full-field optical coherence microscopy (FF-OCM) platform for biological applications is of prime focus today. In this paper, we report a cost-effective Linnik type FF-OCM system with a single broadband white light emitting diode (WLED) (470-850 nm, λ0 = 630 nm) light source in conjunction with a derivative-based image reconstruction algorithm. Sequential 2-D multiple phase-shifted interferograms were obtained by moving the sample stage using piezoelectric transducer and recorded via a 2-D charge complementary oxide semiconductor camera. Subsequently, the en-face sectional images were reconstructed using a derivative-based algorithm. The measured axial and lateral resolutions along with interferometric phase stability of the system were 0.9 μm, 1.4 μm, and 11 mrad, respectively. The advantages of the derivative-based algorithm over 4-step phase shifted method were demonstrated using USAF target and onion as test samples. Moreover, studies via the present system of the microstructure of skin epidermis in mouse ear with melanoma showed a significant deterioration from normal mouse skin in stratum corneum of the epidermis. This is the first study of the application of a derivative-based algorithm for biological samples in FF-OCM wherein WLED is used as a light source. The present system is stable, compact, and cost-effective compared to the conventional FF-OCM systems and provides comparable spatial resolution.
AB - Developing cost-effective and fast detection methods in full-field optical coherence microscopy (FF-OCM) platform for biological applications is of prime focus today. In this paper, we report a cost-effective Linnik type FF-OCM system with a single broadband white light emitting diode (WLED) (470-850 nm, λ0 = 630 nm) light source in conjunction with a derivative-based image reconstruction algorithm. Sequential 2-D multiple phase-shifted interferograms were obtained by moving the sample stage using piezoelectric transducer and recorded via a 2-D charge complementary oxide semiconductor camera. Subsequently, the en-face sectional images were reconstructed using a derivative-based algorithm. The measured axial and lateral resolutions along with interferometric phase stability of the system were 0.9 μm, 1.4 μm, and 11 mrad, respectively. The advantages of the derivative-based algorithm over 4-step phase shifted method were demonstrated using USAF target and onion as test samples. Moreover, studies via the present system of the microstructure of skin epidermis in mouse ear with melanoma showed a significant deterioration from normal mouse skin in stratum corneum of the epidermis. This is the first study of the application of a derivative-based algorithm for biological samples in FF-OCM wherein WLED is used as a light source. The present system is stable, compact, and cost-effective compared to the conventional FF-OCM systems and provides comparable spatial resolution.
KW - Full-field optical coherence microscopy (FF-OCM)
KW - derivative-based algorithms
KW - imaging system
KW - melanoma
KW - white light emitting diodes
UR - http://www.scopus.com/inward/record.url?scp=85011308127&partnerID=8YFLogxK
U2 - 10.1109/JPHOT.2017.2686978
DO - 10.1109/JPHOT.2017.2686978
M3 - Article
AN - SCOPUS:85011308127
SN - 1943-0655
VL - 9
JO - IEEE Photonics Journal
JF - IEEE Photonics Journal
IS - 2
M1 - 7886316
ER -