4D spatiotemporal modulation of biomolecules distribution in anisotropic corrugated microwrinkles via electrically manipulated microcapsules within hierarchical hydrogel for spinal cord regeneration

Min Yu Chiang, Hung Wei Cheng, Yu Chih Lo, Wei Chun Wang, Shwu Jen Chang, Chu Hsun Cheng, Yu Chang Lin, Huai En Lu, Ming Wen Sue, Nien-Ti Tsou, Yu Chun Lo, Ssu Ju Li, Chao Hung Kuo, You Yin Chen, Wei Chen Huang, San Yuan Chen*

*此作品的通信作者

    研究成果: Article同行評審

    摘要

    Although traditional 3D scaffolds or biomimetic hydrogels have been used for tissue engineering and regenerative medicine, soft tissue microenvironment usually has a highly anisotropic structure and a dynamically controllable deformation with various biomolecule distribution. In this study, we developed a hierarchical hybrid gelatin methacrylate-microcapsule hydrogel (HGMH) with Neurotrophin-3(NT-3)-loaded PLGA microcapsules to fabricate anisotropic structure with patterned NT-3 distribution (demonstrated as striped and triangular patterns) by dielectrophoresis (DEP). The HGMH provides a dynamic biomimetic sinuate-microwrinkles change with NT-3 spatial gradient and 2-stage time-dependent distribution, which was further simulated using a 3D finite element model. As demonstrated, in comparison with striped-patterned hydrogel, the triangular-patterned HGMH with highly anisotropic array of microcapsules exhibits remarkably spatial NT-3 gradient distributions that can not only guide neural stem cells (NSCs) migration but also facilitate spinal cord injury regeneration. This approach to construct hierarchical 4D hydrogel system via an electromicrofluidic platform demonstrates the potential for building various biomimetic soft scaffolds in vitro tailed to real soft tissues.

    原文English
    文章編號120762
    期刊Biomaterials
    271
    DOIs
    出版狀態Published - 四月 2021

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