3D pH measurement for cell culture using CT scan

Nobuya Higashino; Toshio Takayama; Hiroaki Ito; Mitsuhiro Horade; Chia-Hung Tsai; Makoto Kaneko

doi:10.1109/MHS.2017.8305213

3D pH measurement for cell culture using CT scan

Nobuya Higashino^*, Toshio Takayama, Hiroaki Ito, Mitsuhiro Horade, Chia-Hung Tsai, Makoto Kaneko

^*此作品的通信作者

機械工程學系

研究成果: Conference contribution › 同行評審

摘要

In cell culture, the pH of culture liquid is one of the most important conditions. However, culture liquid may have pH-gradient due to the activity of cells and change of the environment. In order to confirm the existence of pH-gradient, we propose a method for 3D pH measurement using the color change of phenol red. Using the principle of CT scan, we can measure pH-gradient without contacting culture liquid, and thus, decrease the possibility of contamination. In this paper, we consider the influence of light refraction by a cylindrical vessel, and show that we can measure local pH of culture liquid by light absorbance.

原文	English
主出版物標題	MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science
發行者	Institute of Electrical and Electronics Engineers Inc.
頁面	1-3
頁數	3
ISBN（電子）	9781538633144
DOIs	https://doi.org/10.1109/MHS.2017.8305213
出版狀態	Published - 28 2月 2018
事件	28th International Symposium on Micro-NanoMechatronics and Human Science, MHS 2017 - Nagoya, 日本持續時間: 3 12月 2017 → 6 12月 2017

出版系列

名字	MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science
卷	2018-January

Conference

Conference	28th International Symposium on Micro-NanoMechatronics and Human Science, MHS 2017
國家/地區	日本
城市	Nagoya
期間	3/12/17 → 6/12/17

存取文件

10.1109/MHS.2017.8305213

其它文件與鏈接

Link to publication in Scopus

引用此

Higashino, N., Takayama, T., Ito, H., Horade, M., Tsai, C.-H., & Kaneko, M. (2018). 3D pH measurement for cell culture using CT scan. 於 MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science (頁 1-3). (MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science; 卷 2018-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MHS.2017.8305213

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title = "3D pH measurement for cell culture using CT scan",

abstract = "In cell culture, the pH of culture liquid is one of the most important conditions. However, culture liquid may have pH-gradient due to the activity of cells and change of the environment. In order to confirm the existence of pH-gradient, we propose a method for 3D pH measurement using the color change of phenol red. Using the principle of CT scan, we can measure pH-gradient without contacting culture liquid, and thus, decrease the possibility of contamination. In this paper, we consider the influence of light refraction by a cylindrical vessel, and show that we can measure local pH of culture liquid by light absorbance.",

author = "Nobuya Higashino and Toshio Takayama and Hiroaki Ito and Mitsuhiro Horade and Chia-Hung Tsai and Makoto Kaneko",

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doi = "10.1109/MHS.2017.8305213",

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note = "28th International Symposium on Micro-NanoMechatronics and Human Science, MHS 2017 ; Conference date: 03-12-2017 Through 06-12-2017",

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Higashino, N, Takayama, T, Ito, H, Horade, M, Tsai, C-H & Kaneko, M 2018, 3D pH measurement for cell culture using CT scan. 於 MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science. MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science, 卷 2018-January, Institute of Electrical and Electronics Engineers Inc., 頁 1-3, 28th International Symposium on Micro-NanoMechatronics and Human Science, MHS 2017, Nagoya, 日本, 3/12/17. https://doi.org/10.1109/MHS.2017.8305213

3D pH measurement for cell culture using CT scan. / Higashino, Nobuya; Takayama, Toshio; Ito, Hiroaki 等.
MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science. Institute of Electrical and Electronics Engineers Inc., 2018. p. 1-3 (MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science; 卷 2018-January).

研究成果: Conference contribution › 同行評審

TY - GEN

T1 - 3D pH measurement for cell culture using CT scan

AU - Higashino, Nobuya

AU - Takayama, Toshio

AU - Ito, Hiroaki

AU - Horade, Mitsuhiro

AU - Tsai, Chia-Hung

AU - Kaneko, Makoto

PY - 2018/2/28

Y1 - 2018/2/28

N2 - In cell culture, the pH of culture liquid is one of the most important conditions. However, culture liquid may have pH-gradient due to the activity of cells and change of the environment. In order to confirm the existence of pH-gradient, we propose a method for 3D pH measurement using the color change of phenol red. Using the principle of CT scan, we can measure pH-gradient without contacting culture liquid, and thus, decrease the possibility of contamination. In this paper, we consider the influence of light refraction by a cylindrical vessel, and show that we can measure local pH of culture liquid by light absorbance.

AB - In cell culture, the pH of culture liquid is one of the most important conditions. However, culture liquid may have pH-gradient due to the activity of cells and change of the environment. In order to confirm the existence of pH-gradient, we propose a method for 3D pH measurement using the color change of phenol red. Using the principle of CT scan, we can measure pH-gradient without contacting culture liquid, and thus, decrease the possibility of contamination. In this paper, we consider the influence of light refraction by a cylindrical vessel, and show that we can measure local pH of culture liquid by light absorbance.

UR - http://www.scopus.com/inward/record.url?scp=85050466382&partnerID=8YFLogxK

U2 - 10.1109/MHS.2017.8305213

DO - 10.1109/MHS.2017.8305213

M3 - Conference contribution

AN - SCOPUS:85050466382

T3 - MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science

SP - 1

EP - 3

BT - MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 28th International Symposium on Micro-NanoMechatronics and Human Science, MHS 2017

Y2 - 3 December 2017 through 6 December 2017

ER -

Higashino N, Takayama T, Ito H, Horade M, Tsai CH, Kaneko M. 3D pH measurement for cell culture using CT scan. 於 MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science. Institute of Electrical and Electronics Engineers Inc. 2018. p. 1-3. (MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science). doi: 10.1109/MHS.2017.8305213

3D pH measurement for cell culture using CT scan

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Conference

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