3‐Benzoyl‐2‐naphthaldehyde, a new fluorogenic reagent for microcolumn liquid chromatography–laser‐induced fluorescence detection of amino acids

You‐Zung ‐Z Hsieh, Stephen C. Beale, Donald Wiesler, Milos Novotny*

*此作品的通信作者

研究成果: Article同行評審

13 引文 斯高帕斯(Scopus)

摘要

3‐Benzoyl‐2‐naphthaldehyde (BNA) was synthesized and characterized as a precolumn derivatization reagent for fluorescence detection. The detection limits for standard amino acids derivatized with BNA are in the femtogram range. The optimum reaction conditions for derivatization and spectral features of BNA‐derivatized amino acids was investigated. The standard amino acid derivatives were adequately resolved by microcolumn liquid chromatography. A minute quantity (646 fmol) of trypsin was hydrolyzed to demonstrate analysis by microcolumn liquid chromatography with laser‐induced fluorescence detection.

原文English
頁(從 - 到)96-100
頁數5
期刊Journal of Microcolumn Separations
1
發行號2
DOIs
出版狀態Published - 1989

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