TY - GEN
T1 - Zeolite magnetic nano/micro-particles for adsorption, delivery and release of photodynamic dyes
AU - Hovhannisyan, Vladimir
AU - Siposova, Katarina
AU - Musatov, Andrej
AU - Mitroova, Zuzana
AU - Chen, Shean Jen
PY - 2019
Y1 - 2019
N2 - Adsorption of photodynamic active dyes (hypericin, chlorin e6, methylene blue, ALA and fluorescein) and they release from clinoptilolite type of zeolite (CZ) in the presence of biomolecules, such as collagen, albumin and hemoglobin, were quantitatively investigated by absorption and fluorescence spectrometry. Using multiphoton microscopy, we demonstrated that effective inducing of two-photon excited luminescence and second harmonic generation signals in nano/micro-particles of CZ by femtosecond near-infrared laser excitation can be successfully utilized in multiphoton imaging of CZ particles and the dye adsorption processes. In addition, CZ magnetic (MCZ) particles were fabricated and proposed as a promising material for drug delivery and controlled release in biological systems. It is shown that the temperature of the aqueous suspension of MCZ of 2 cm3 can be increased by 7 ºC for 3 minutes by an induction heater. Furthermore, it was demonstrated that MCZ particles have strong inhibition and destruction effects on insulin and lysozyme fibrillization, and the protein amyloid formation is inhibited in a dose-dependent manner. Optical spectrometry and multiphoton microscopy are effective approaches that may reveal potential of magnetic zeolites in drug delivery and biomedical imaging of cancer photodynamic therapy and hyperthermia.
AB - Adsorption of photodynamic active dyes (hypericin, chlorin e6, methylene blue, ALA and fluorescein) and they release from clinoptilolite type of zeolite (CZ) in the presence of biomolecules, such as collagen, albumin and hemoglobin, were quantitatively investigated by absorption and fluorescence spectrometry. Using multiphoton microscopy, we demonstrated that effective inducing of two-photon excited luminescence and second harmonic generation signals in nano/micro-particles of CZ by femtosecond near-infrared laser excitation can be successfully utilized in multiphoton imaging of CZ particles and the dye adsorption processes. In addition, CZ magnetic (MCZ) particles were fabricated and proposed as a promising material for drug delivery and controlled release in biological systems. It is shown that the temperature of the aqueous suspension of MCZ of 2 cm3 can be increased by 7 ºC for 3 minutes by an induction heater. Furthermore, it was demonstrated that MCZ particles have strong inhibition and destruction effects on insulin and lysozyme fibrillization, and the protein amyloid formation is inhibited in a dose-dependent manner. Optical spectrometry and multiphoton microscopy are effective approaches that may reveal potential of magnetic zeolites in drug delivery and biomedical imaging of cancer photodynamic therapy and hyperthermia.
KW - Drug delivery
KW - Magnetic nanoparticles
KW - Multiphoton microscopy
KW - Zeolite
UR - http://www.scopus.com/inward/record.url?scp=85084493858&partnerID=8YFLogxK
U2 - 10.1117/12.2526924
DO - 10.1117/12.2526924
M3 - Conference contribution
AN - SCOPUS:85074292501
T3 - Optics InfoBase Conference Papers
BT - European Conference on Biomedical Optics, ECBO_2019
PB - OSA - The Optical Society
T2 - European Conference on Biomedical Optics, ECBO_2019
Y2 - 23 June 2019 through 25 June 2019
ER -
