Wireless near-infrared spectroscopy system for determining brain hemoglobin levels in laboratory animals

Jinn Rung Kuo, Ming Hsien Chang, Che Chuan Wang, Chung Ching Chio, Jhi Joung Wang, Bor-Shyh Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Traumatic brain injury (TBI) is usually caused by brain shaking or impact. It can affect normal brain function and may even lead to disability or death. However, there are very few studies on the associated physiologic changes in humans or animals. In this study, a non-invasive, wireless multi-channel near-infrared spectroscopy (NIRS) was developed to continuously monitor the concentration change of oxyhemoglobin (HbO2), deoxyhemoglobin (HbR), and total hemoglobin (HbT) to elucidate changes in the physiological state of the brain during and after different strength impaction. The triphenyltetrazolium chloride (TTC) staining was also used to monitor changes of infarction volume after different strength impaction. The results indicated that the concentration changes of HbO2 and HbT, and the changes of infarction volumes were significantly related to the impact strength. In conclusion, the status of TBI can be clinically evaluated by detecting HbO2 and HbT changes. The system proposed here is stable, accurate, non-invasive, and mostly important wireless which can easily be used for TBI study.

Original languageEnglish
Pages (from-to)204-209
Number of pages6
JournalJournal of Neuroscience Methods
Volume214
Issue number2
DOIs
StatePublished - 5 Apr 2013

Keywords

  • Deoxyhemoglobin
  • Near-infrared spectroscopy
  • Oxyhemoglobin
  • Total hemoglobin
  • Traumatic brain injury
  • Triphenyltetrazolium chloride

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