Abstract
We have demonstrated a widely-tunable femtosecond fiber source between 770-1180 nm enabled by self-phase modulation, and the wide spectral coverage is suitable for most of two-photon fluorescence microscopy applications. Based on femtosecond Yb:fiber laser, we also compared spectral broadening in different dispersion regimes using different photonic crystal fibers. We managed to maximize the self-phase modulated feature from the broadened spectra while avoiding unwanted nonlinear temporal trapping. The optimization of fiber selection and laser input conditions led to a wide tunability down to below 800nm region, which is the most commonly used two-photon excitation wavelength for many intrinsic fluorescent labels in biological tissues. We believe this fiber-based femtosecond source can be a relatively cost-effective and robust solution for most of two-photon fluorescence microscopy applications.
| Original language | English |
|---|---|
| Title of host publication | Frontiers in Ultrafast Optics |
| Subtitle of host publication | Biomedical, Scientific, and Industrial Applications XX |
| Editors | Peter R. Herman, Michel Meunier, Roberto Osellame |
| Publisher | SPIE |
| ISBN (Electronic) | 9781510633032 |
| DOIs | |
| State | Published - 2020 |
| Event | Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XX 2020 - San Francisco, United States Duration: 1 Feb 2020 → 4 Feb 2020 |
Publication series
| Name | Proceedings of SPIE - The International Society for Optical Engineering |
|---|---|
| Volume | 11270 |
| ISSN (Print) | 0277-786X |
| ISSN (Electronic) | 1996-756X |
Conference
| Conference | Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XX 2020 |
|---|---|
| Country/Territory | United States |
| City | San Francisco |
| Period | 1/02/20 → 4/02/20 |
Keywords
- Nonlinear fiber optics
- Self-phase-modulation-enabled spectral selection
- Two-photon microscopy