Water molecules are active during the primary photoreaction of bacteriorhodopsin

Wolfgang B. Fischer, Kenneth J. Rothschild*

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

2 Scopus citations


FT-IR difference spectra of the primary photoreaction of bacteriorhodopsin, the light driven proton pump from Halobacterium halobium, are presented with data above 3500 cm-1. Samples were hydrated in H2O, H218O and D2O in order to distinguish vibrational bands due to water. In the bR→K difference spectrum, evidence is found for a water molecule which undergoes a strengthening in hydrogen bonding. In contrast, during the K→L and L→M steps of the photocycle one or more additional water molecules undergo a weakening in hydrogen bonding.

Original languageEnglish
Pages (from-to)118-119
Number of pages2
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 31 Jan 1994
Event9th International Conference on Fourier Transform Spectroscopy 1993 - Calgary, Canada
Duration: 23 Aug 199327 Aug 1993


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