TY - JOUR
T1 - Water molecules are active during the primary photoreaction of bacteriorhodopsin
AU - Fischer, Wolfgang B.
AU - Rothschild, Kenneth J.
N1 - Publisher Copyright:
© 1994 SPIE. All rights reserved.
PY - 1994/1/31
Y1 - 1994/1/31
N2 - FT-IR difference spectra of the primary photoreaction of bacteriorhodopsin, the light driven proton pump from Halobacterium halobium, are presented with data above 3500 cm-1. Samples were hydrated in H2O, H218O and D2O in order to distinguish vibrational bands due to water. In the bR→K difference spectrum, evidence is found for a water molecule which undergoes a strengthening in hydrogen bonding. In contrast, during the K→L and L→M steps of the photocycle one or more additional water molecules undergo a weakening in hydrogen bonding.
AB - FT-IR difference spectra of the primary photoreaction of bacteriorhodopsin, the light driven proton pump from Halobacterium halobium, are presented with data above 3500 cm-1. Samples were hydrated in H2O, H218O and D2O in order to distinguish vibrational bands due to water. In the bR→K difference spectrum, evidence is found for a water molecule which undergoes a strengthening in hydrogen bonding. In contrast, during the K→L and L→M steps of the photocycle one or more additional water molecules undergo a weakening in hydrogen bonding.
UR - http://www.scopus.com/inward/record.url?scp=0042617088&partnerID=8YFLogxK
U2 - 10.1117/12.166685
DO - 10.1117/12.166685
M3 - Conference article
AN - SCOPUS:0042617088
SN - 0277-786X
VL - 2089
SP - 118
EP - 119
JO - Proceedings of SPIE - The International Society for Optical Engineering
JF - Proceedings of SPIE - The International Society for Optical Engineering
T2 - 9th International Conference on Fourier Transform Spectroscopy 1993
Y2 - 23 August 1993 through 27 August 1993
ER -