FT-IR difference spectra of the primary photoreaction of bacteriorhodopsin, the light driven proton pump from Halobacterium halobium, are presented with data above 3500 cm-1. Samples were hydrated in H2O, H218O and D2O in order to distinguish vibrational bands due to water. In the bR→K difference spectrum, evidence is found for a water molecule which undergoes a strengthening in hydrogen bonding. In contrast, during the K→L and L→M steps of the photocycle one or more additional water molecules undergo a weakening in hydrogen bonding.
|Number of pages
|Proceedings of SPIE - The International Society for Optical Engineering
|Published - 31 Jan 1994
|9th International Conference on Fourier Transform Spectroscopy 1993 - Calgary, Canada
Duration: 23 Aug 1993 → 27 Aug 1993