Using siRNA to uncover novel oncogenic signaling pathways.

Jin Mei Lai*, Chi Ying F. Huang, Chang Han Chen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Tumor invasion and metastasis are the primary causes of cancer patient mortality, underscoring the need for identification of novel genes and signaling pathways that mediate these prognosis-determining phenomena. To identify and characterize novel lung adenocarcinoma genes associated with lung cancer progression, we created a bioinformatics-based approach that focuses on human cell-cycle-regulated genes that have evolved only in higher organisms but not in lower eukaryotic cells. In siRNA experiments in lung cancer cells, FLJ10540 was identified as one of several novel targets involved in cell migration and invasion. Here, we demonstrate that PI3K inhibition affects FLJ10540-mediated cell migration and invasion and further, that FLJ10540 knockdown ablates AKT-Ser(473) phosphorylation. Taken together, these findings indicate that the FLJ10540/PI3K/AKT pathway may harbor new therapeutic targets for treating invasive lung adenocarcinoma.

Original languageEnglish
Pages (from-to)231-242
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
StatePublished - 2010


Dive into the research topics of 'Using siRNA to uncover novel oncogenic signaling pathways.'. Together they form a unique fingerprint.

Cite this