Using cationic polyurethane-short branch PEI as microRNA-driven nano-delivery system for stem cell differentiation

Chian-Shiu Chien; Chien-Ying Wang; Yi-Ping Yang; Shih-Jie Chou; Yu-Ling Ko; Fu-Ting Tsai; Wen-Chung Yu; Chia-Ching Chang; Jong-Yuh Cherng; Meng-Yin Yang

doi:10.1097/JCMA.0000000000000272

Using cationic polyurethane-short branch PEI as microRNA-driven nano-delivery system for stem cell differentiation

Chian-Shiu Chien, Chien-Ying Wang, Yi-Ping Yang, Shih-Jie Chou, Yu-Ling Ko, Fu-Ting Tsai, Wen-Chung Yu, Chia-Ching Chang, Jong-Yuh Cherng, Meng-Yin Yang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Background:

Non-viral gene delivery, such as using biodegradable polyurethane short-branch polyethylenimine (PU-PEI), has been considered a potentially safer gene delivery system in comparison to conventional virus systems.

Methods:

The polycationization of DNA complexes protects DNA from nuclease degradation, and these DNA complexes are nanoscale in size to enter the cell through endocytosis.

Results:

Due to the net positive surface charge of the cell, these polyplexes efficiently bind to the cell through electrostatic interactions with negatively charged membrane components. Cationic PU-PEI has been shown to be non-cytotoxic and has a high transfection efficiency, making it a practical gene delivery material in diseases.

Conclusion:

We developed a PU-PEI nanomedicine-based platform to efficiently deliver microRNA in promoting differentiation capacity of stem cells, especially on induced pluripotent stem cells.

Original language	English
Pages (from-to)	367-370
Number of pages	4
Journal	Journal of the Chinese Medical Association
Volume	83
Issue number	4
DOIs	https://doi.org/10.1097/JCMA.0000000000000272
State	Published - Apr 2020

Keywords

Induced pluripotent stem cells
Non-viral gene delivery
Polyurethane short-branch polyethylenimine
TRANSFECTION
PROLIFERATION

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10.1097/JCMA.0000000000000272

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title = "Using cationic polyurethane-short branch PEI as microRNA-driven nano-delivery system for stem cell differentiation",

abstract = "Background:Non-viral gene delivery, such as using biodegradable polyurethane short-branch polyethylenimine (PU-PEI), has been considered a potentially safer gene delivery system in comparison to conventional virus systems.Methods:The polycationization of DNA complexes protects DNA from nuclease degradation, and these DNA complexes are nanoscale in size to enter the cell through endocytosis.Results:Due to the net positive surface charge of the cell, these polyplexes efficiently bind to the cell through electrostatic interactions with negatively charged membrane components. Cationic PU-PEI has been shown to be non-cytotoxic and has a high transfection efficiency, making it a practical gene delivery material in diseases.Conclusion:We developed a PU-PEI nanomedicine-based platform to efficiently deliver microRNA in promoting differentiation capacity of stem cells, especially on induced pluripotent stem cells.",

keywords = "Induced pluripotent stem cells, Non-viral gene delivery, Polyurethane short-branch polyethylenimine, TRANSFECTION, PROLIFERATION",

author = "Chian-Shiu Chien and Chien-Ying Wang and Yi-Ping Yang and Shih-Jie Chou and Yu-Ling Ko and Fu-Ting Tsai and Wen-Chung Yu and Chia-Ching Chang and Jong-Yuh Cherng and Meng-Yin Yang",

year = "2020",

month = apr,

doi = "10.1097/JCMA.0000000000000272",

language = "English",

volume = "83",

pages = "367--370",

journal = "Journal of the Chinese Medical Association",

issn = "1726-4901",

publisher = "Elsevier Taiwan LLC",

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TY - JOUR

T1 - Using cationic polyurethane-short branch PEI as microRNA-driven nano-delivery system for stem cell differentiation

AU - Chien, Chian-Shiu

AU - Wang, Chien-Ying

AU - Yang, Yi-Ping

AU - Chou, Shih-Jie

AU - Ko, Yu-Ling

AU - Tsai, Fu-Ting

AU - Yu, Wen-Chung

AU - Chang, Chia-Ching

AU - Cherng, Jong-Yuh

AU - Yang, Meng-Yin

PY - 2020/4

Y1 - 2020/4

N2 - Background:Non-viral gene delivery, such as using biodegradable polyurethane short-branch polyethylenimine (PU-PEI), has been considered a potentially safer gene delivery system in comparison to conventional virus systems.Methods:The polycationization of DNA complexes protects DNA from nuclease degradation, and these DNA complexes are nanoscale in size to enter the cell through endocytosis.Results:Due to the net positive surface charge of the cell, these polyplexes efficiently bind to the cell through electrostatic interactions with negatively charged membrane components. Cationic PU-PEI has been shown to be non-cytotoxic and has a high transfection efficiency, making it a practical gene delivery material in diseases.Conclusion:We developed a PU-PEI nanomedicine-based platform to efficiently deliver microRNA in promoting differentiation capacity of stem cells, especially on induced pluripotent stem cells.

AB - Background:Non-viral gene delivery, such as using biodegradable polyurethane short-branch polyethylenimine (PU-PEI), has been considered a potentially safer gene delivery system in comparison to conventional virus systems.Methods:The polycationization of DNA complexes protects DNA from nuclease degradation, and these DNA complexes are nanoscale in size to enter the cell through endocytosis.Results:Due to the net positive surface charge of the cell, these polyplexes efficiently bind to the cell through electrostatic interactions with negatively charged membrane components. Cationic PU-PEI has been shown to be non-cytotoxic and has a high transfection efficiency, making it a practical gene delivery material in diseases.Conclusion:We developed a PU-PEI nanomedicine-based platform to efficiently deliver microRNA in promoting differentiation capacity of stem cells, especially on induced pluripotent stem cells.

KW - Induced pluripotent stem cells

KW - Non-viral gene delivery

KW - Polyurethane short-branch polyethylenimine

KW - TRANSFECTION

KW - PROLIFERATION

U2 - 10.1097/JCMA.0000000000000272

DO - 10.1097/JCMA.0000000000000272

M3 - Article

C2 - 32101899

SN - 1726-4901

VL - 83

SP - 367

EP - 370

JO - Journal of the Chinese Medical Association

JF - Journal of the Chinese Medical Association

IS - 4

ER -

Using cationic polyurethane-short branch PEI as microRNA-driven nano-delivery system for stem cell differentiation

Abstract

Keywords

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