Unravelling 3D Dynamics and Hydrodynamics during Incorporation of Dielectric Particles to an Optical Trapping Site

Boris Louis, Chih Hao Huang, Rafael Camacho, Ivan G. Scheblykin, Teruki Sugiyama*, Tetsuhiro Kudo, Marc Melendez, Rafael Delgado-Buscalioni, Hiroshi Masuhara*, Johan Hofkens*, Roger Bresoli-Obach*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Mapping of the spatial and temporal motion of particles inside an optical field is critical for understanding and further improvement of the 3D spatio-temporal control over their optical trapping dynamics. However, it is not trivial to capture the 3D motion, and most imaging systems only capture a 2D projection of the 3D motion, in which the information about the axial movement is not directly available. In this work, we resolve the 3D incorporation trajectories of 200 nm fluorescent polystyrene particles in an optical trapping site under different optical experimental conditions using a recently developed widefield multiplane microscope (imaging volume of 50 × 50 × 4 μm3). The particles are gathered at the focus following some preferential 3D channels that show a shallow cone distribution. We demonstrate that the radial and the axial flow speed components depend on the axial distance from the focus, which is directly related to the scattering/gradient optical forces. While particle velocities and trajectories are mainly determined by the trapping laser profile, they cannot be completely explained without considering collective effects resulting from hydrodynamic forces.

Original languageEnglish
Pages (from-to)3797-3808
Number of pages12
JournalACS Nano
Issue number4
StatePublished - 28 Feb 2023


  • 3D imaging
  • hydrodynamics
  • multiplane widefield microscopy
  • optical field
  • optical trapping
  • particle tracking


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