Ultraviolet spectrum identification of emodin in rabbit plasma by HPLC and its pharmacokinetics application

T. H. Tsai, C. F. Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

A simple and sensitive high-performance liquid chromatography method for the determination of emodin in rabbit plasma has been developed. Up to 0.1 ml of plasma containing emodin was deproteined by acetonitrile, which contained an internal standard (indomethacin). The supernatant was injected into a LiChrosphere RP-18 column using acetonitrile-water (70:30, v/v, pH 2.5-2.8, adjust with phosphoric acid) as the mobile phase and UV detection at 221 nm, followed by UV spectrum identification (between 200 and 380 nm) with a photodiode array detector. The method is rapid, easily reproduced, selective and sensitive. It was applied to pharmacokinetic studies of emodin in rabbit, after a 2 mg kg-1 intravenous administration. A biphase phenomenon with a rapid distribution followed by a slower elimination phase was observed from the plasma concentration-time curve. Compartmental analysis yielded on a two-compartment model.

Original languageEnglish
Pages (from-to)53-56
Number of pages4
JournalAsia Pacific Journal of Pharmacology
Volume7
Issue number1
StatePublished - 1992

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