Ultrasensitive and specific two-photon fluorescence detection of hypochlorous acid by a lysosome-targeting fluorescent probe for cell imaging

Guan Syuan Wu, Natesan Thirumalaivasan, Tsung Chieh Lin, Shu-Pao Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Hypochlorous acid (HOCl) is involved in numerous cellular processes, such as pathogen response, immune regulation, and anti-inflammation. Consequently, the development of HOCl detection at the cellular level has been an important issue in investigating the dynamic distributions of HOCl. Herein, a fluorescent probe, Lyso-NA, containing a HOCl-reactive aminophenol group and a lysosomal-targeting morpholine group, has been effectively designed for detecting lysosomal HOCl. The reaction of Lyso-NA with HOCl induces the oxidation of aminophenol and accompanied by a 136-fold fluorescence enhancement. The detection limit is found at 13 nM. The fluorescence enhancement is accomplished through the suppression of twisted intramolecular charge transfer (TICT). With morpholine, the probe Lyso-NA shows the great lysosomal targetable ability for imaging endogenous lysosomal HOCl in living cells and tissues by two-photon microscopy, providing an opportunity to monitor HOCl in the lysosomes for understanding its biological functions.

Original languageEnglish
Article number113545
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume190
DOIs
StatePublished - 25 Oct 2020

Keywords

  • Fluorescent sensor
  • Hypochlorous acid
  • Lysosome
  • Two-photon effect

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