Two-stage optical trapping and assembling of protein at air/solution interface

Po Wei Yi, Wei Hsiang Chiu, Shuichi Toyouchi, Roger Bresolí-Obach, Johan Hofkens, Eri Chatani, Yoichiroh Hosokawa, Teruki Sugiyama, Hiroshi Masuhara

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Optical trapping at interfaces has recently gained relevance due to the expansion of optical potential far away from the focus, especially for proteins where submillimeter structures have been described. Initially, lysozyme clusters are trapped as a shallow layer at the surface, becoming thicker with irradiation time. Nonetheless, overcoming a concentration threshold, lysozyme clusters inside the solution are collected and transported toward the focus, invading the lysozyme layer, which results in a border between them, although no concentration jump is detected. This two-stage optical trapping occurs due to the long-range interaction originating from the focus.

Original languageEnglish
Article number025501
JournalApplied Physics Express
Issue number2
StatePublished - Feb 2023


  • lysozyme
  • multiplane microscope
  • optical trapping
  • polystyrene microparticle
  • protein cluster
  • solution surface


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