Tumor marker detection by aptamer-functionalized graphene oxide

Ming Yu Lin; Yen Pei Lu; Alexandru Mihai Grumezescu; Fu Han Ho; Ying Hsien Kao; Yuh-Shyong Yang; Chih Hui Yang

doi:10.2174/1385272811317020008

Tumor marker detection by aptamer-functionalized graphene oxide

Ming Yu Lin, Yen Pei Lu, Alexandru Mihai Grumezescu, Fu Han Ho, Ying Hsien Kao, Yuh-Shyong Yang, Chih Hui Yang^*

^*Corresponding author for this work

Department of Biological Science and Technology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Interaction of the single-stranded DNA (ssDNA) aptamers and proteins was investigated on graphene oxide (GO)-based biosensors. GO was applied for biological applications because of its unique properties, which include water dispensability, exclusive selection of ssDNA, and fluorescence-quenching effects. In this study, aptamer-functionalized GO sheets of nanometer-scale thickness and micrometer-scale dimension were developed. Based on a simple mixed-and-measured strategy, platelet-derived growth factors (PDGF), the tumor marker of ovarian cancer, triggered aptamers conformation switched and deliberated aptamers from GO surface according to thermal dynamic analysis. By functionalizing with various aptamers on GO, the strategy could provide designed functions on GO-based biosensors, bioreactors, and other devices.

Original language	English
Pages (from-to)	132-136
Number of pages	5
Journal	Current Organic Chemistry
Volume	17
Issue number	2
DOIs	https://doi.org/10.2174/1385272811317020008
State	Published - 25 Jun 2013

Keywords

Aptamers
Biosensors
Functionalization
Graphene oxide
Platelet-derived growth factors
Tumor marker

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2174/1385272811317020008

Cite this

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title = "Tumor marker detection by aptamer-functionalized graphene oxide",

abstract = "Interaction of the single-stranded DNA (ssDNA) aptamers and proteins was investigated on graphene oxide (GO)-based biosensors. GO was applied for biological applications because of its unique properties, which include water dispensability, exclusive selection of ssDNA, and fluorescence-quenching effects. In this study, aptamer-functionalized GO sheets of nanometer-scale thickness and micrometer-scale dimension were developed. Based on a simple mixed-and-measured strategy, platelet-derived growth factors (PDGF), the tumor marker of ovarian cancer, triggered aptamers conformation switched and deliberated aptamers from GO surface according to thermal dynamic analysis. By functionalizing with various aptamers on GO, the strategy could provide designed functions on GO-based biosensors, bioreactors, and other devices.",

keywords = "Aptamers, Biosensors, Functionalization, Graphene oxide, Platelet-derived growth factors, Tumor marker",

author = "Lin, {Ming Yu} and Lu, {Yen Pei} and Grumezescu, {Alexandru Mihai} and Ho, {Fu Han} and Kao, {Ying Hsien} and Yuh-Shyong Yang and Yang, {Chih Hui}",

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doi = "10.2174/1385272811317020008",

language = "English",

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T1 - Tumor marker detection by aptamer-functionalized graphene oxide

AU - Lin, Ming Yu

AU - Lu, Yen Pei

AU - Grumezescu, Alexandru Mihai

AU - Ho, Fu Han

AU - Kao, Ying Hsien

AU - Yang, Yuh-Shyong

AU - Yang, Chih Hui

PY - 2013/6/25

Y1 - 2013/6/25

N2 - Interaction of the single-stranded DNA (ssDNA) aptamers and proteins was investigated on graphene oxide (GO)-based biosensors. GO was applied for biological applications because of its unique properties, which include water dispensability, exclusive selection of ssDNA, and fluorescence-quenching effects. In this study, aptamer-functionalized GO sheets of nanometer-scale thickness and micrometer-scale dimension were developed. Based on a simple mixed-and-measured strategy, platelet-derived growth factors (PDGF), the tumor marker of ovarian cancer, triggered aptamers conformation switched and deliberated aptamers from GO surface according to thermal dynamic analysis. By functionalizing with various aptamers on GO, the strategy could provide designed functions on GO-based biosensors, bioreactors, and other devices.

AB - Interaction of the single-stranded DNA (ssDNA) aptamers and proteins was investigated on graphene oxide (GO)-based biosensors. GO was applied for biological applications because of its unique properties, which include water dispensability, exclusive selection of ssDNA, and fluorescence-quenching effects. In this study, aptamer-functionalized GO sheets of nanometer-scale thickness and micrometer-scale dimension were developed. Based on a simple mixed-and-measured strategy, platelet-derived growth factors (PDGF), the tumor marker of ovarian cancer, triggered aptamers conformation switched and deliberated aptamers from GO surface according to thermal dynamic analysis. By functionalizing with various aptamers on GO, the strategy could provide designed functions on GO-based biosensors, bioreactors, and other devices.

KW - Aptamers

KW - Biosensors

KW - Functionalization

KW - Graphene oxide

KW - Platelet-derived growth factors

KW - Tumor marker

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Tumor marker detection by aptamer-functionalized graphene oxide

Abstract

Keywords

UN SDGs

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