Transgenic mice expressing surface markers for IFN-γ and IL-4 producing cells

Shie Liang Hsieh; Nien Jung Chen; Kristin Tarbell; Nan Shih Liao; Yein Gei Lai; Kun Hsiung Lee; Kin Mu Lee; Shinn Chih Wu; Huey Kang Sytwu; Shou Hwa Han; Hugh McDevitt

doi:10.1016/S0161-5890(00)00052-3

Transgenic mice expressing surface markers for IFN-γ and IL-4 producing cells

Shie Liang Hsieh, Nien Jung Chen, Kristin Tarbell, Nan Shih Liao, Yein Gei Lai, Kun Hsiung Lee, Kin Mu Lee, Shinn Chih Wu, Huey Kang Sytwu, Shou Hwa Han, Hugh McDevitt^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The detection of T(H)1-type and T(H)2-type cells directly in situ would be of great value in the study of T(H) development and function in vivo. Transgenic mice expressing human Thy1 and mouse Thy1.1 under the control of the murine IFN-γ and IL-4 promoters, respectively, have been generated. The hThy1⁺ cells represent (with some temporal lag) most of the IFN-γ-producing CD4⁺ T-cells, while the mThy1.1⁺ cells represent only a percentage of IL-4 secreting cells. This may be due to mono-allelic expression of the IFN-γ and IL-4 genes. Since permeabilization is not required for the detection of the transgenic surface markers, these transgenic mice can facilitate the detection of T(H)1-type and T(H)2-type cells by flow cytometry with surface immunofluorescent staining. These surface markers should permit isolation of viable cells according to their T(H) type for adoptive transfer experiments, and may serve as a model system for tracing the development of T(H)1 and T(H)2-type cells in vivo. (C) 2000 Elsevier Science Ltd.

Original language	English
Pages (from-to)	281-293
Number of pages	13
Journal	Molecular Immunology
Volume	37
Issue number	6
DOIs	https://doi.org/10.1016/S0161-5890(00)00052-3
State	Published - 2000

Keywords

T lymphocytes
T(H)1/T(H)2
Transgenic

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10.1016/S0161-5890(00)00052-3

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@article{e2b0164815cd4a1aa4ee40770af2c485,

title = "Transgenic mice expressing surface markers for IFN-γ and IL-4 producing cells",

abstract = "The detection of T(H)1-type and T(H)2-type cells directly in situ would be of great value in the study of T(H) development and function in vivo. Transgenic mice expressing human Thy1 and mouse Thy1.1 under the control of the murine IFN-γ and IL-4 promoters, respectively, have been generated. The hThy1+ cells represent (with some temporal lag) most of the IFN-γ-producing CD4+ T-cells, while the mThy1.1+ cells represent only a percentage of IL-4 secreting cells. This may be due to mono-allelic expression of the IFN-γ and IL-4 genes. Since permeabilization is not required for the detection of the transgenic surface markers, these transgenic mice can facilitate the detection of T(H)1-type and T(H)2-type cells by flow cytometry with surface immunofluorescent staining. These surface markers should permit isolation of viable cells according to their T(H) type for adoptive transfer experiments, and may serve as a model system for tracing the development of T(H)1 and T(H)2-type cells in vivo. (C) 2000 Elsevier Science Ltd.",

keywords = "T lymphocytes, T(H)1/T(H)2, Transgenic",

author = "Hsieh, {Shie Liang} and Chen, {Nien Jung} and Kristin Tarbell and Liao, {Nan Shih} and Lai, {Yein Gei} and Lee, {Kun Hsiung} and Lee, {Kin Mu} and Wu, {Shinn Chih} and Sytwu, {Huey Kang} and Han, {Shou Hwa} and Hugh McDevitt",

note = "Funding Information: This work was supported by the National Institute of Health, Bethesda, MD and National Health Research Institute, Taiwan, Republic of China (DOH85-HR-508). We would like to thank Dr Jack Silver for providing both the human Thy1 and murine Thy1.1 genomic clones, Dr Naoko Arai for providing the murine IL-4 promoter, and Dr Howard Fox for providing the murine IFN-γ promoter. Special thanks also go to Chi-Hung Lin, Hsu-Chin Chen and Weber Chen for their technical assistance.",

year = "2000",

doi = "10.1016/S0161-5890(00)00052-3",

language = "English",

volume = "37",

pages = "281--293",

journal = "Molecular Immunology",

issn = "0161-5890",

publisher = "Elsevier Ltd",

number = "6",

}

TY - JOUR

T1 - Transgenic mice expressing surface markers for IFN-γ and IL-4 producing cells

AU - Hsieh, Shie Liang

AU - Chen, Nien Jung

AU - Tarbell, Kristin

AU - Liao, Nan Shih

AU - Lai, Yein Gei

AU - Lee, Kun Hsiung

AU - Lee, Kin Mu

AU - Wu, Shinn Chih

AU - Sytwu, Huey Kang

AU - Han, Shou Hwa

AU - McDevitt, Hugh

N1 - Funding Information: This work was supported by the National Institute of Health, Bethesda, MD and National Health Research Institute, Taiwan, Republic of China (DOH85-HR-508). We would like to thank Dr Jack Silver for providing both the human Thy1 and murine Thy1.1 genomic clones, Dr Naoko Arai for providing the murine IL-4 promoter, and Dr Howard Fox for providing the murine IFN-γ promoter. Special thanks also go to Chi-Hung Lin, Hsu-Chin Chen and Weber Chen for their technical assistance.

PY - 2000

Y1 - 2000

N2 - The detection of T(H)1-type and T(H)2-type cells directly in situ would be of great value in the study of T(H) development and function in vivo. Transgenic mice expressing human Thy1 and mouse Thy1.1 under the control of the murine IFN-γ and IL-4 promoters, respectively, have been generated. The hThy1+ cells represent (with some temporal lag) most of the IFN-γ-producing CD4+ T-cells, while the mThy1.1+ cells represent only a percentage of IL-4 secreting cells. This may be due to mono-allelic expression of the IFN-γ and IL-4 genes. Since permeabilization is not required for the detection of the transgenic surface markers, these transgenic mice can facilitate the detection of T(H)1-type and T(H)2-type cells by flow cytometry with surface immunofluorescent staining. These surface markers should permit isolation of viable cells according to their T(H) type for adoptive transfer experiments, and may serve as a model system for tracing the development of T(H)1 and T(H)2-type cells in vivo. (C) 2000 Elsevier Science Ltd.

AB - The detection of T(H)1-type and T(H)2-type cells directly in situ would be of great value in the study of T(H) development and function in vivo. Transgenic mice expressing human Thy1 and mouse Thy1.1 under the control of the murine IFN-γ and IL-4 promoters, respectively, have been generated. The hThy1+ cells represent (with some temporal lag) most of the IFN-γ-producing CD4+ T-cells, while the mThy1.1+ cells represent only a percentage of IL-4 secreting cells. This may be due to mono-allelic expression of the IFN-γ and IL-4 genes. Since permeabilization is not required for the detection of the transgenic surface markers, these transgenic mice can facilitate the detection of T(H)1-type and T(H)2-type cells by flow cytometry with surface immunofluorescent staining. These surface markers should permit isolation of viable cells according to their T(H) type for adoptive transfer experiments, and may serve as a model system for tracing the development of T(H)1 and T(H)2-type cells in vivo. (C) 2000 Elsevier Science Ltd.

KW - T lymphocytes

KW - T(H)1/T(H)2

KW - Transgenic

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U2 - 10.1016/S0161-5890(00)00052-3

DO - 10.1016/S0161-5890(00)00052-3

M3 - Article

C2 - 11000402

AN - SCOPUS:0033824989

SN - 0161-5890

VL - 37

SP - 281

EP - 293

JO - Molecular Immunology

JF - Molecular Immunology

IS - 6

ER -

Transgenic mice expressing surface markers for IFN-γ and IL-4 producing cells

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Keywords

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