Transgenic mice expressing surface markers for IFN-γ and IL-4 producing cells

Shie Liang Hsieh, Nien Jung Chen, Kristin Tarbell, Nan Shih Liao, Yein Gei Lai, Kun Hsiung Lee, Kin Mu Lee, Shinn Chih Wu, Huey Kang Sytwu, Shou Hwa Han, Hugh McDevitt*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


The detection of T(H)1-type and T(H)2-type cells directly in situ would be of great value in the study of T(H) development and function in vivo. Transgenic mice expressing human Thy1 and mouse Thy1.1 under the control of the murine IFN-γ and IL-4 promoters, respectively, have been generated. The hThy1+ cells represent (with some temporal lag) most of the IFN-γ-producing CD4+ T-cells, while the mThy1.1+ cells represent only a percentage of IL-4 secreting cells. This may be due to mono-allelic expression of the IFN-γ and IL-4 genes. Since permeabilization is not required for the detection of the transgenic surface markers, these transgenic mice can facilitate the detection of T(H)1-type and T(H)2-type cells by flow cytometry with surface immunofluorescent staining. These surface markers should permit isolation of viable cells according to their T(H) type for adoptive transfer experiments, and may serve as a model system for tracing the development of T(H)1 and T(H)2-type cells in vivo. (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)281-293
Number of pages13
JournalMolecular Immunology
Issue number6
StatePublished - 2000


  • T lymphocytes
  • T(H)1/T(H)2
  • Transgenic


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