Transcriptional regulation of the gene coding for mouse thymosin β-4

Y. Su*, H. I. Haian, S. L. Chang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Thymosin β-4 (Tβ4), a 43 residue acidic peptide, is one of the major G-actin sequestering proteins that distributes widely in vertebrates, Tβ4 gene, previously identified as the interferonstimulated gene 6-26 (ISG6-26), whose expression is shown to be regulated mainly at the level of transcription by differentiationinducing agents, hormones, or cytokines in a variety of cells and tissues. To elucidate the transcriptional mechanism of the Tβ4 gene, we transfected fusion genes consisting of various portions of the Tβ4 5' flanking and intronic sequences that were linked to a chloramphenicol acetyltransferase (CAT) reporter gene transiently into NIH3T3 cells. We found an intronic region (+132 to +1030) strongly activates, whereas a 5' flanking region (-882 to -285) suppresses, the expression of the reporter gene. This result indicates the complexity in the regulation of mouse Tβ4 gene expression.

Original languageEnglish
Pages (from-to)A1070
JournalFASEB Journal
Volume11
Issue number9
StatePublished - 1997

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