TY - JOUR
T1 - Time-resolved autofluorescence spectroscopy for classifying normal and premalignant oral tissues
AU - Chen, Hsin Ming
AU - Chiang, Chun Pin
AU - You, Chun
AU - Hsiao, Tzu-Chien
AU - Wang, Chih Yu
PY - 2005/7
Y1 - 2005/7
N2 - Background and Objectives: Time-resolved autofluorescence spectroscopy has been used for effectively distinguishing normal tissues from precancers and cancers in various organs. The aim of this study was to find out the possibility of using time-resolved autofluorescence spectroscopy to differentiate normal oral mucosa (NOM) from oral premalignant lesions including verrucous hyperplasia (VH), epithelial hyperplasia (EH), and epithelial dysplasia (ED). Study Design/Materials and Methods: Time-resolved autofluorescence spectra at 633 nm under 410-nm excitation were recorded for 15 VH, 9 EH, 14 ED, and 38 NOM samples. The two-component lifetimes of the obtained curves were calculated, and a Fisher's discriminant analysis (FDA) was employed for distinguishing these tissue samples. Results: After two-component lifetimes for all samples being calculated, a two-dimensional scatter plot was developed, in which 76 oral tissue samples were separated into three groups by FDA. With a leave-one-out method, the FDA algorithm gave an accuracy rate of 93% for ED, of 75% for VH and EH, and of 100% for NOM samples. In addition, all oral premalignant lesions (including VH, EH, and ED) could be distinguished from NOM samples by this FDA algorithm. Conclusions: We conclude that time-resolved autofluorescence spectroscopy at 633 nm under 410-nm excitation, based on two-component lifetime calculation and FDA, is a very sensitive technique for in vivo diagnosis of oral pre-malignant lesions.
AB - Background and Objectives: Time-resolved autofluorescence spectroscopy has been used for effectively distinguishing normal tissues from precancers and cancers in various organs. The aim of this study was to find out the possibility of using time-resolved autofluorescence spectroscopy to differentiate normal oral mucosa (NOM) from oral premalignant lesions including verrucous hyperplasia (VH), epithelial hyperplasia (EH), and epithelial dysplasia (ED). Study Design/Materials and Methods: Time-resolved autofluorescence spectra at 633 nm under 410-nm excitation were recorded for 15 VH, 9 EH, 14 ED, and 38 NOM samples. The two-component lifetimes of the obtained curves were calculated, and a Fisher's discriminant analysis (FDA) was employed for distinguishing these tissue samples. Results: After two-component lifetimes for all samples being calculated, a two-dimensional scatter plot was developed, in which 76 oral tissue samples were separated into three groups by FDA. With a leave-one-out method, the FDA algorithm gave an accuracy rate of 93% for ED, of 75% for VH and EH, and of 100% for NOM samples. In addition, all oral premalignant lesions (including VH, EH, and ED) could be distinguished from NOM samples by this FDA algorithm. Conclusions: We conclude that time-resolved autofluorescence spectroscopy at 633 nm under 410-nm excitation, based on two-component lifetime calculation and FDA, is a very sensitive technique for in vivo diagnosis of oral pre-malignant lesions.
KW - Fluorescence lifetime
KW - Oral premalignance
KW - Time-resolved autofluorescence spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=23344445932&partnerID=8YFLogxK
U2 - 10.1002/lsm.20192
DO - 10.1002/lsm.20192
M3 - Article
C2 - 15954122
AN - SCOPUS:23344445932
SN - 0196-8092
VL - 37
SP - 37
EP - 45
JO - Lasers in Surgery and Medicine
JF - Lasers in Surgery and Medicine
IS - 1
ER -