The roles of propeptide in maturation and secretion of Npr protease from Streptomyces

Su Chih Chang, Poa Chun Chang, Yan-Hwa Wu Lee*

*Corresponding author for this work

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2 Scopus citations


The cloned npr gene of Streptomyces cacaoi encodes a 60-kDa protein (prepro-Npr) consisting of a typical secretory signal peptide, a propeptide (22 kDa), and the 35-kDa mature metalloprotease (Npr). The maturation of Npr occurs extracellularly via an autocatalytic cleavage of the secreted intermediate pro-Npr (Chang, P. C., and Lee, Y.-H. W. (1992) J. Biol. Chem. 267, 3952-3958). In this study, we investigated the roles of the propeptide in the maturation and secretion of Npr. Partial deletion of the propeptide region while leaving the signal peptide and the mature Npr sequence intact all led to abolishment of Npr activity and caused concomitant slight and transient accumulation of low molecular weight forms of Npr or pro-Npr derivatives extracellularly. The intact propeptide and its truncated form alone could be secreted into the medium if their NH2 termini were directly fused with the signal peptide sequence of Npr. However, similar fusion of the mature protease domain to the signal peptide without the propeptide sequence completely abolished the Npr production intracellularly and extracellularly. All these results demonstrate that the propeptide plays an important role in maturation and secretion of Npr protease, as in the case of α-lytic protease and subtilisin. In addition, our data suggest that an intact propeptide region is essential for the formation of mature active Npr, but not for the secretion of Npr and its derivatives. This distinguishes the maturation and secretion of S. cacaoi Npr from those of other propeptide-containing bacterial serine proteases and thermolysin-like protease.

Original languageEnglish
Pages (from-to)3548-3554
Number of pages7
JournalJournal of Biological Chemistry
Issue number5
StatePublished - 4 Feb 1994


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