The postnatal development of the gabaa/benzodiazepine receptor in the rat red nucleus

Y. S. Fu*, G. F. Tseng, H. S. Yin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The development of the GABAA/Benzodiazepine receptor (GABAAR) in the red nucleus was studied using 3H-flunitrazepam (FNZ) as the probe. Saturation binding assay showed that the Bmax of the ligand to the membranes of the nucleus increased from 0.50 ± 0.04 nmol/mg protein at postnatal day 4, to 0.71 ± 0.1 and 0.78 ± 0.08 at day 7 and day 10. At day 20 the Bmax decreased to a level near day 4 and persisted until day 40. However, the affinity of 3H-FNZ to the receptor remained quite constant. At least 4 proteins of 51kD, 53kD, 59kD and 62kD in the nucleus were labeled by 3H-FNZ, as revealed from photoaffinity binding and SDS-PAGE. The labeling of 53kD, 59kD and 62kD was high at earlier ages than day 10, whereas the 51kD was predominent from day 10 to day 40. Receptor binding autoradiography of the nucleus also showed that the most dense labeling was seen around day 10. The early transient increase in the GABAAR of the red nucleus may indicate the plasticity of the nucleus in response to environmental changes after birth.

Original languageEnglish
Pages (from-to)267-280
Number of pages14
JournalJournal of Receptor and Signal Transduction Research
Volume14
Issue number3-4
DOIs
StatePublished - 1994

Fingerprint

Dive into the research topics of 'The postnatal development of the gabaa/benzodiazepine receptor in the rat red nucleus'. Together they form a unique fingerprint.

Cite this