The effect of PEG-5K grafting level and particle size on tumor accumulation and cellular uptake

Chun Liang Lo; Meng Han Chou; Pei Lin Lu; I. Wen Lo; Yi Ting Chiang; Shang Yu Hung; Chieh Yu Yang; Shuian Yin Lin; Shiaw Pyng Wey; Jem Mau Lo; Ging Ho Hsiue

doi:10.1016/j.ijpharm.2013.08.045

The effect of PEG-5K grafting level and particle size on tumor accumulation and cellular uptake

Chun Liang Lo^*, Meng Han Chou, Pei Lin Lu, I. Wen Lo, Yi Ting Chiang, Shang Yu Hung, Chieh Yu Yang, Shuian Yin Lin, Shiaw Pyng Wey, Jem Mau Lo, Ging Ho Hsiue

^*Corresponding author for this work

Department of Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

PEG-modified gold nanoparticles (PEG-modified GNs) with diameters of 40 nm and 70 nm were prepared to elucidate the effect of extent of PEG (M.W. 5000) grafting and particle size on tumor accumulation and cellular uptake. Flow cytometry reveals that cellular uptake is strongly related to the size of PEG-modified GNs, rather than the extent of PEG-5K grafting level. Cytotoxicity analysis based on the intracellular release of drugs showed that the 70 nm PEG-modified GNs have the higher cytotoxicity, beccause of their greater cellular uptake. Also, particle size, rather than PEG-5K grafting level affects tumor accumulation. However, PEG-5K grafting level significantly affects the accumulation of particles in the liver and spleen. This finding is important in determining the proper PEG-5K grafting level and particle size for designing nano-medicines.

Original language	English
Pages (from-to)	424-431
Number of pages	8
Journal	International Journal of Pharmaceutics
Volume	456
Issue number	2
DOIs	https://doi.org/10.1016/j.ijpharm.2013.08.045
State	Published - 2013

Keywords

Biodistribution
Cellular uptake
Gold nanoparticles
Polyethylene glycol
Surface modification

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10.1016/j.ijpharm.2013.08.045

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title = "The effect of PEG-5K grafting level and particle size on tumor accumulation and cellular uptake",

abstract = "PEG-modified gold nanoparticles (PEG-modified GNs) with diameters of 40 nm and 70 nm were prepared to elucidate the effect of extent of PEG (M.W. 5000) grafting and particle size on tumor accumulation and cellular uptake. Flow cytometry reveals that cellular uptake is strongly related to the size of PEG-modified GNs, rather than the extent of PEG-5K grafting level. Cytotoxicity analysis based on the intracellular release of drugs showed that the 70 nm PEG-modified GNs have the higher cytotoxicity, beccause of their greater cellular uptake. Also, particle size, rather than PEG-5K grafting level affects tumor accumulation. However, PEG-5K grafting level significantly affects the accumulation of particles in the liver and spleen. This finding is important in determining the proper PEG-5K grafting level and particle size for designing nano-medicines.",

keywords = "Biodistribution, Cellular uptake, Gold nanoparticles, Polyethylene glycol, Surface modification",

author = "Lo, {Chun Liang} and Chou, {Meng Han} and Lu, {Pei Lin} and Lo, {I. Wen} and Chiang, {Yi Ting} and Hung, {Shang Yu} and Yang, {Chieh Yu} and Lin, {Shuian Yin} and Wey, {Shiaw Pyng} and Lo, {Jem Mau} and Hsiue, {Ging Ho}",

note = "Funding Information: The authors would like to thank the National Science Council of the Republic of China, Taiwan for financially supporting this work (NSC 101-2120-M-033-001 and NSC 101-2627-E-010-001 ). TTY biopharm Co. Ltd. (Taiwan) is appreciated for kindly providing the doxorubicin hydrochloride.",

year = "2013",

doi = "10.1016/j.ijpharm.2013.08.045",

language = "English",

volume = "456",

pages = "424--431",

journal = "International Journal of Pharmaceutics",

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T1 - The effect of PEG-5K grafting level and particle size on tumor accumulation and cellular uptake

AU - Lo, Chun Liang

AU - Chou, Meng Han

AU - Lu, Pei Lin

AU - Lo, I. Wen

AU - Chiang, Yi Ting

AU - Hung, Shang Yu

AU - Yang, Chieh Yu

AU - Lin, Shuian Yin

AU - Wey, Shiaw Pyng

AU - Lo, Jem Mau

AU - Hsiue, Ging Ho

N1 - Funding Information: The authors would like to thank the National Science Council of the Republic of China, Taiwan for financially supporting this work (NSC 101-2120-M-033-001 and NSC 101-2627-E-010-001 ). TTY biopharm Co. Ltd. (Taiwan) is appreciated for kindly providing the doxorubicin hydrochloride.

PY - 2013

Y1 - 2013

N2 - PEG-modified gold nanoparticles (PEG-modified GNs) with diameters of 40 nm and 70 nm were prepared to elucidate the effect of extent of PEG (M.W. 5000) grafting and particle size on tumor accumulation and cellular uptake. Flow cytometry reveals that cellular uptake is strongly related to the size of PEG-modified GNs, rather than the extent of PEG-5K grafting level. Cytotoxicity analysis based on the intracellular release of drugs showed that the 70 nm PEG-modified GNs have the higher cytotoxicity, beccause of their greater cellular uptake. Also, particle size, rather than PEG-5K grafting level affects tumor accumulation. However, PEG-5K grafting level significantly affects the accumulation of particles in the liver and spleen. This finding is important in determining the proper PEG-5K grafting level and particle size for designing nano-medicines.

AB - PEG-modified gold nanoparticles (PEG-modified GNs) with diameters of 40 nm and 70 nm were prepared to elucidate the effect of extent of PEG (M.W. 5000) grafting and particle size on tumor accumulation and cellular uptake. Flow cytometry reveals that cellular uptake is strongly related to the size of PEG-modified GNs, rather than the extent of PEG-5K grafting level. Cytotoxicity analysis based on the intracellular release of drugs showed that the 70 nm PEG-modified GNs have the higher cytotoxicity, beccause of their greater cellular uptake. Also, particle size, rather than PEG-5K grafting level affects tumor accumulation. However, PEG-5K grafting level significantly affects the accumulation of particles in the liver and spleen. This finding is important in determining the proper PEG-5K grafting level and particle size for designing nano-medicines.

KW - Biodistribution

KW - Cellular uptake

KW - Gold nanoparticles

KW - Polyethylene glycol

KW - Surface modification

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VL - 456

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EP - 431

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

IS - 2

ER -

The effect of PEG-5K grafting level and particle size on tumor accumulation and cellular uptake

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