The association of ATG16L1 variations with clinical phenotypes of adult-onset still’s disease

Wei Ting Hung, Shuen Iu Hung, Yi Ming Chen, Chia Wei Hsieh, Hsin Hua Chen, Kuo Tung Tang, Der Yuan Chen*, Tsuo Hung Lan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Adult-onset Still’s disease (AOSD) is a rare autoinflammatory disease, which has elevated autophagosome levels regulated by autophagy-related gene (ATG) expression. We investigated the associations of ATG polymorphisms with AOSD susceptibility, clinical manifestations, and disease course. The six-candidate single-nucleotide polymorphisms (SNPs) involved in autophagy were genotyped using direct sequencing on samples from 129 AOSD patients and 129 healthy participants. The differentially expressed gene products were quantified using PCR and ELISA. Significant linkage disequilibrium was noted in three SNPs of autophagy-related 16-like 1 (ATG16L1) gene (rs10210302, rs2241880, and rs1045100). Although the AA/CC/TT haplotype of ATG16L1 was not associated with the susceptibility of our AOSD patients compared with other haplotypes, those carrying this haplotype had lower mRNA expression levels of LC3-II, reflecting by autophagosome formation (p = 0.026). Patients carrying AA/CC/TT haplotype also have a significantly higher proportion of skin rash and a lower proportion of arthritis compared with other haplotypes. The AA/CC/TT haplotype was significantly associated with systemic pattern (odds ratio, 3.25; 95% confidence interval, 1.15–9.14; p = 0.026). In summary, the AA/CC/TT haplotype encoded lower levels of autophagosome formation and was associated with a higher proportion of skin rash and systemic pattern of AOSD compared with other haplotypes.

Original languageEnglish
Article number904
JournalGenes
Volume12
Issue number6
DOIs
StatePublished - Jun 2021

Keywords

  • Adult-onset Still’s disease
  • ATG16L1
  • Autophagy
  • Haplotype
  • Single-nucleotide polymorphism

Fingerprint

Dive into the research topics of 'The association of ATG16L1 variations with clinical phenotypes of adult-onset still’s disease'. Together they form a unique fingerprint.

Cite this