The antiapoptotic protein Mcl-1 is essential for the survival of neutrophils but not macrophages

Ivan Dzhagalov, Ashley St. John, You Wen He*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

226 Scopus citations

Abstract

The antiapoptotic protein Mcl-1, a member of the Bcl-2 family, plays critical roles in promoting the survival of lymphocytes and hematopoietic stem cells. Although previous studies have implicated Mcl-1 in regulating the survival of neutrophils and macrophages, the in vivo function of Mcl-1 in these 2 cell lineages remained unclear. To address this, we have generated mice conditionally lacking Mcl-1 expression in neutrophils and macrophages. We show that Mcl-1 conditional knockout mice had a severe defect in neutrophil survival, whereas macrophage survival was normal. The granulocyte compartment in the blood, spleen, and bone marrow of Mcl-1 conditional knockout mice exhibited an approximately 2- to 3-fold higher apoptotic rate than control cells. In contrast, resting and activated macrophages from Mcl-1-deficient mice exhibited normal survival and contained up-regulated expression of Bcl-2 and Bcl-x L. These data suggest that Mcl-1 plays a nonredundant role in promoting the survival of neutrophils but not macrophages.

Original languageEnglish
Pages (from-to)1620-1626
Number of pages7
JournalBlood
Volume109
Issue number4
DOIs
StatePublished - 15 Feb 2007

Fingerprint

Dive into the research topics of 'The antiapoptotic protein Mcl-1 is essential for the survival of neutrophils but not macrophages'. Together they form a unique fingerprint.

Cite this