Targeting of miR-31/96/182 to the Numb gene during head and neck oncogenesis

Chung Hsien Chou; Hsi Feng Tu; Shou Yen Kao; Chun Yu Fan Chiang; Chung Ji Liu; Kuo Wei Chang; Shu Chun Lin

doi:10.1002/hed.25063

Targeting of miR-31/96/182 to the Numb gene during head and neck oncogenesis

Chung Hsien Chou, Hsi Feng Tu, Shou Yen Kao, Chun Yu Fan Chiang, Chung Ji Liu, Kuo Wei Chang, Shu Chun Lin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Background: MicroRNAs (miRNAs) play crucial roles in head and neck squamous cell carcinoma (HNSCC). This study investigates whether miR-31, miR-96, and miR-182 are involved in targeting Numb during HNSCC. Methods: The expression of miR-31/96/182 in tumor tissues was analyzed. Reporter assay, knockdown, expression, and oncogenic analysis were carried out in cell lines. Results: Upregulation of miR-31/96/182 was detected in tumor tissues. In addition, advanced tumors showed higher expression levels of these miRNAs. The expression of these miRNAs was upregulated after treatment with areca ingredients (P <.01 or P <.001). These miRNAs directly targeted the 3′ untranslated region (UTR) sequence of the Numb gene. An increased migration and invasion of HNSCC cells was associated with the exogenous expression of miR-31/96/182 (P <.01 or P <.001), and this was reverted by expression of Numb. Conclusion: This study provides new evidence demonstrating that there is frequent and concordant upregulation of miR-31, miR-96, and miR-182 during HNSCC and these miRNAs co-target Numb.

Original language	English
Pages (from-to)	808-817
Number of pages	10
Journal	Head and Neck
Volume	40
Issue number	4
DOIs	https://doi.org/10.1002/hed.25063
State	Published - Apr 2018

Keywords

Numb
carcinoma
miR-182
miR-31
miR-96
mouth

Access to Document

10.1002/hed.25063

Cite this

@article{0b30379c92ab4c4fb1bb7b5f13a37c97,

title = "Targeting of miR-31/96/182 to the Numb gene during head and neck oncogenesis",

abstract = "Background: MicroRNAs (miRNAs) play crucial roles in head and neck squamous cell carcinoma (HNSCC). This study investigates whether miR-31, miR-96, and miR-182 are involved in targeting Numb during HNSCC. Methods: The expression of miR-31/96/182 in tumor tissues was analyzed. Reporter assay, knockdown, expression, and oncogenic analysis were carried out in cell lines. Results: Upregulation of miR-31/96/182 was detected in tumor tissues. In addition, advanced tumors showed higher expression levels of these miRNAs. The expression of these miRNAs was upregulated after treatment with areca ingredients (P <.01 or P <.001). These miRNAs directly targeted the 3′ untranslated region (UTR) sequence of the Numb gene. An increased migration and invasion of HNSCC cells was associated with the exogenous expression of miR-31/96/182 (P <.01 or P <.001), and this was reverted by expression of Numb. Conclusion: This study provides new evidence demonstrating that there is frequent and concordant upregulation of miR-31, miR-96, and miR-182 during HNSCC and these miRNAs co-target Numb.",

keywords = "Numb, carcinoma, miR-182, miR-31, miR-96, mouth",

author = "Chou, {Chung Hsien} and Tu, {Hsi Feng} and Kao, {Shou Yen} and Chiang, {Chun Yu Fan} and Liu, {Chung Ji} and Chang, {Kuo Wei} and Lin, {Shu Chun}",

note = "Publisher Copyright: {\textcopyright} 2018 Wiley Periodicals, Inc.",

year = "2018",

month = apr,

doi = "10.1002/hed.25063",

language = "English",

volume = "40",

pages = "808--817",

journal = "Head and Neck",

issn = "1043-3074",

publisher = "John Wiley & Sons Inc.",

number = "4",

}

TY - JOUR

T1 - Targeting of miR-31/96/182 to the Numb gene during head and neck oncogenesis

AU - Chou, Chung Hsien

AU - Tu, Hsi Feng

AU - Kao, Shou Yen

AU - Chiang, Chun Yu Fan

AU - Liu, Chung Ji

AU - Chang, Kuo Wei

AU - Lin, Shu Chun

PY - 2018/4

Y1 - 2018/4

N2 - Background: MicroRNAs (miRNAs) play crucial roles in head and neck squamous cell carcinoma (HNSCC). This study investigates whether miR-31, miR-96, and miR-182 are involved in targeting Numb during HNSCC. Methods: The expression of miR-31/96/182 in tumor tissues was analyzed. Reporter assay, knockdown, expression, and oncogenic analysis were carried out in cell lines. Results: Upregulation of miR-31/96/182 was detected in tumor tissues. In addition, advanced tumors showed higher expression levels of these miRNAs. The expression of these miRNAs was upregulated after treatment with areca ingredients (P <.01 or P <.001). These miRNAs directly targeted the 3′ untranslated region (UTR) sequence of the Numb gene. An increased migration and invasion of HNSCC cells was associated with the exogenous expression of miR-31/96/182 (P <.01 or P <.001), and this was reverted by expression of Numb. Conclusion: This study provides new evidence demonstrating that there is frequent and concordant upregulation of miR-31, miR-96, and miR-182 during HNSCC and these miRNAs co-target Numb.

AB - Background: MicroRNAs (miRNAs) play crucial roles in head and neck squamous cell carcinoma (HNSCC). This study investigates whether miR-31, miR-96, and miR-182 are involved in targeting Numb during HNSCC. Methods: The expression of miR-31/96/182 in tumor tissues was analyzed. Reporter assay, knockdown, expression, and oncogenic analysis were carried out in cell lines. Results: Upregulation of miR-31/96/182 was detected in tumor tissues. In addition, advanced tumors showed higher expression levels of these miRNAs. The expression of these miRNAs was upregulated after treatment with areca ingredients (P <.01 or P <.001). These miRNAs directly targeted the 3′ untranslated region (UTR) sequence of the Numb gene. An increased migration and invasion of HNSCC cells was associated with the exogenous expression of miR-31/96/182 (P <.01 or P <.001), and this was reverted by expression of Numb. Conclusion: This study provides new evidence demonstrating that there is frequent and concordant upregulation of miR-31, miR-96, and miR-182 during HNSCC and these miRNAs co-target Numb.

KW - Numb

KW - carcinoma

KW - miR-182

KW - miR-31

KW - miR-96

KW - mouth

UR - http://www.scopus.com/inward/record.url?scp=85043572836&partnerID=8YFLogxK

U2 - 10.1002/hed.25063

DO - 10.1002/hed.25063

M3 - Article

C2 - 29356167

AN - SCOPUS:85043572836

SN - 1043-3074

VL - 40

SP - 808

EP - 817

JO - Head and Neck

JF - Head and Neck

IS - 4

ER -

Targeting of miR-31/96/182 to the Numb gene during head and neck oncogenesis

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this