Synthesis, characterization, and application of superparamagnetic iron oxide nanoprobes for extrapulmonary tuberculosis detection

Chun Nin Lee; Li Hsuan Chiu; Chia Lang Fang; Shauh Der Yeh; Chun S. Zuo; Shih Ching Chen; Li Kuo Kuo; Yun-Ming Wang; Wen Fu Thomas Lai

doi:10.3791/58227

Synthesis, characterization, and application of superparamagnetic iron oxide nanoprobes for extrapulmonary tuberculosis detection

Chun Nin Lee, Li Hsuan Chiu, Chia Lang Fang, Shauh Der Yeh, Chun S. Zuo, Shih Ching Chen, Li Kuo Kuo, Yun-Ming Wang^*, Wen Fu Thomas Lai^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

A molecular imaging probe comprising superparamagnetic iron oxide (SPIO) nanoparticles and Mycobacterium tuberculosis surface antibody (MtbsAb) was synthesized to enhance imaging sensitivity for extrapulmonary tuberculosis (ETB). An SPIO nanoprobe was synthesized and conjugated with MtbsAb. The purified SPIO-MtbsAb nanoprobe was characterized using TEM and NMR. To determine the targeting ability of the probe, SPIO-MtbsAb nanoprobes were incubated with Mtb for in vitro imaging assays and injected into Mtb-inoculated mice for in vivo investigation with magnetic resonance (MR). The contrast enhancement reduction on magnetic resonance imaging (MRI) of Mtb and THP1 cells showed proportional to the SPIO-MtbsAb nanoprobe concentration. After 30 min of intravenous SPIO-MtbsAb nanoprobe injection into Mtb-infected mice, the signal intensity of the granulomatous site was enhanced by 14-fold in the T2-weighted MR images compared with that in mice receiving PBS injection. The MtbsAb nanoprobes can be used as a novel modality for ETB detection.

Original language	English
Article number	e58227
Pages (from-to)	1-9
Number of pages	9
Journal	Journal of Visualized Experiments
Volume	2020
Issue number	156
DOIs	https://doi.org/10.3791/58227
State	Published - 16 Feb 2020

Keywords

Berlin blue stain
Diagnosis
Extrapulmonary
Issue 156
Medicine
Molecular imaging
Mycobaterium tuberculosis
Nanoprobe
Ziehl-Neelsen stain

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{c5f8fe1bb3b0439e8e13db28246bb5de,

title = "Synthesis, characterization, and application of superparamagnetic iron oxide nanoprobes for extrapulmonary tuberculosis detection",

abstract = "A molecular imaging probe comprising superparamagnetic iron oxide (SPIO) nanoparticles and Mycobacterium tuberculosis surface antibody (MtbsAb) was synthesized to enhance imaging sensitivity for extrapulmonary tuberculosis (ETB). An SPIO nanoprobe was synthesized and conjugated with MtbsAb. The purified SPIO-MtbsAb nanoprobe was characterized using TEM and NMR. To determine the targeting ability of the probe, SPIO-MtbsAb nanoprobes were incubated with Mtb for in vitro imaging assays and injected into Mtb-inoculated mice for in vivo investigation with magnetic resonance (MR). The contrast enhancement reduction on magnetic resonance imaging (MRI) of Mtb and THP1 cells showed proportional to the SPIO-MtbsAb nanoprobe concentration. After 30 min of intravenous SPIO-MtbsAb nanoprobe injection into Mtb-infected mice, the signal intensity of the granulomatous site was enhanced by 14-fold in the T2-weighted MR images compared with that in mice receiving PBS injection. The MtbsAb nanoprobes can be used as a novel modality for ETB detection.",

keywords = "Berlin blue stain, Diagnosis, Extrapulmonary, Issue 156, Medicine, Molecular imaging, Mycobaterium tuberculosis, Nanoprobe, Ziehl-Neelsen stain",

author = "Lee, {Chun Nin} and Chiu, {Li Hsuan} and Fang, {Chia Lang} and Yeh, {Shauh Der} and Zuo, {Chun S.} and Chen, {Shih Ching} and Kuo, {Li Kuo} and Yun-Ming Wang and Lai, {Wen Fu Thomas}",

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AU - Lee, Chun Nin

AU - Chiu, Li Hsuan

AU - Fang, Chia Lang

AU - Yeh, Shauh Der

AU - Zuo, Chun S.

AU - Chen, Shih Ching

AU - Kuo, Li Kuo

AU - Wang, Yun-Ming

AU - Lai, Wen Fu Thomas

PY - 2020/2/16

Y1 - 2020/2/16

N2 - A molecular imaging probe comprising superparamagnetic iron oxide (SPIO) nanoparticles and Mycobacterium tuberculosis surface antibody (MtbsAb) was synthesized to enhance imaging sensitivity for extrapulmonary tuberculosis (ETB). An SPIO nanoprobe was synthesized and conjugated with MtbsAb. The purified SPIO-MtbsAb nanoprobe was characterized using TEM and NMR. To determine the targeting ability of the probe, SPIO-MtbsAb nanoprobes were incubated with Mtb for in vitro imaging assays and injected into Mtb-inoculated mice for in vivo investigation with magnetic resonance (MR). The contrast enhancement reduction on magnetic resonance imaging (MRI) of Mtb and THP1 cells showed proportional to the SPIO-MtbsAb nanoprobe concentration. After 30 min of intravenous SPIO-MtbsAb nanoprobe injection into Mtb-infected mice, the signal intensity of the granulomatous site was enhanced by 14-fold in the T2-weighted MR images compared with that in mice receiving PBS injection. The MtbsAb nanoprobes can be used as a novel modality for ETB detection.

AB - A molecular imaging probe comprising superparamagnetic iron oxide (SPIO) nanoparticles and Mycobacterium tuberculosis surface antibody (MtbsAb) was synthesized to enhance imaging sensitivity for extrapulmonary tuberculosis (ETB). An SPIO nanoprobe was synthesized and conjugated with MtbsAb. The purified SPIO-MtbsAb nanoprobe was characterized using TEM and NMR. To determine the targeting ability of the probe, SPIO-MtbsAb nanoprobes were incubated with Mtb for in vitro imaging assays and injected into Mtb-inoculated mice for in vivo investigation with magnetic resonance (MR). The contrast enhancement reduction on magnetic resonance imaging (MRI) of Mtb and THP1 cells showed proportional to the SPIO-MtbsAb nanoprobe concentration. After 30 min of intravenous SPIO-MtbsAb nanoprobe injection into Mtb-infected mice, the signal intensity of the granulomatous site was enhanced by 14-fold in the T2-weighted MR images compared with that in mice receiving PBS injection. The MtbsAb nanoprobes can be used as a novel modality for ETB detection.

KW - Berlin blue stain

KW - Diagnosis

KW - Extrapulmonary

KW - Issue 156

KW - Medicine

KW - Molecular imaging

KW - Mycobaterium tuberculosis

KW - Nanoprobe

KW - Ziehl-Neelsen stain

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Synthesis, characterization, and application of superparamagnetic iron oxide nanoprobes for extrapulmonary tuberculosis detection

Abstract

Keywords

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