Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging

Ruei Yu He; Yuan Deng Su; Keng Chi Cho; Chun Yun Lin; Nan Shan Chang; Chih Han Chang; Shean Jen Chen

doi:10.1364/OE.17.005987

Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging

Ruei Yu He^*, Yuan Deng Su, Keng Chi Cho, Chun Yun Lin, Nan Shan Chang, Chih Han Chang, Shean Jen Chen

^*Corresponding author for this work

Institute of Imaging and Biomedical Photonics

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide fluorescent images of living cell membranes. The proposed microscope with the help of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having a shorter fluorophore lifetime. In comparison with a one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. By combining the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated it's capability for brighter and more contrasted fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.

Original language	English
Pages (from-to)	5987-5997
Number of pages	11
Journal	Optics Express
Volume	17
Issue number	8
DOIs	https://doi.org/10.1364/OE.17.005987
State	Published - 13 Apr 2009

Access to Document

10.1364/OE.17.005987

Cite this

@article{1a83e94d20be47a68fc8998eece69d22,

title = "Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging",

abstract = "A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide fluorescent images of living cell membranes. The proposed microscope with the help of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having a shorter fluorophore lifetime. In comparison with a one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. By combining the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated it's capability for brighter and more contrasted fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.",

author = "He, {Ruei Yu} and Su, {Yuan Deng} and Cho, {Keng Chi} and Lin, {Chun Yun} and Chang, {Nan Shan} and Chang, {Chih Han} and Chen, {Shean Jen}",

year = "2009",

month = apr,

day = "13",

doi = "10.1364/OE.17.005987",

language = "English",

volume = "17",

pages = "5987--5997",

journal = "Optics Express",

issn = "1094-4087",

publisher = "The Optical Society",

number = "8",

}

TY - JOUR

T1 - Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging

AU - He, Ruei Yu

AU - Su, Yuan Deng

AU - Cho, Keng Chi

AU - Lin, Chun Yun

AU - Chang, Nan Shan

AU - Chang, Chih Han

AU - Chen, Shean Jen

PY - 2009/4/13

Y1 - 2009/4/13

N2 - A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide fluorescent images of living cell membranes. The proposed microscope with the help of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having a shorter fluorophore lifetime. In comparison with a one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. By combining the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated it's capability for brighter and more contrasted fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.

AB - A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide fluorescent images of living cell membranes. The proposed microscope with the help of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having a shorter fluorophore lifetime. In comparison with a one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. By combining the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated it's capability for brighter and more contrasted fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.

UR - http://www.scopus.com/inward/record.url?scp=65149084261&partnerID=8YFLogxK

U2 - 10.1364/OE.17.005987

DO - 10.1364/OE.17.005987

M3 - Article

C2 - 19365417

AN - SCOPUS:65149084261

SN - 1094-4087

VL - 17

SP - 5987

EP - 5997

JO - Optics Express

JF - Optics Express

IS - 8

ER -

Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging

Abstract

Access to Document

Other files and links

Fingerprint

Cite this