Surface enhanced FTIR spectroscopy and surface plasmon resonance on biomembranes

Gerald Steiner; Reiner Salzer; Wolfgang Fischer; Christian Kuhne

doi:10.1117/12.384946

Surface enhanced FTIR spectroscopy and surface plasmon resonance on biomembranes

Gerald Steiner^*, Reiner Salzer, Wolfgang Fischer, Christian Kuhne

^*Corresponding author for this work

Institute of Biophotonics

Research output: Contribution to journal › Conference article › peer-review

2 Scopus citations

Abstract

Lipid bilayers containing the nicotinic acetylcholine receptor were investigated by attenuated total reflection (ATR) FTIR spectroscopy and by surface plasmon resonance (SPR). FTIR-ATR spectra reveal structural details of the transmembrane protein. Surface enhanced infrared absorption (SEIRA) provides access to structural details in the close vicinity of the surface of the ATR crystal. Information about the abundance of protein substructures as well as about the orientation of those substructures are obtained. SPR was applied in order to detect changes within the adsorbed biomembrane during admission of a neurotransmitter as acetylcholine. Switching of the ion channel was successfully observed by SPR. This result is a step forward towards highly selective biosensors and bioactuators.

Original language	English
Pages (from-to)	215-221
Number of pages	7
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	3918
DOIs	https://doi.org/10.1117/12.384946
State	Published - 2000
Event	Biomedical Spectroscopy: Vibrational Spectroscopy and other Novel Techniques - San Jose, CA, USA Duration: 26 Jan 2000 → 27 Jan 2000

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title = "Surface enhanced FTIR spectroscopy and surface plasmon resonance on biomembranes",

abstract = "Lipid bilayers containing the nicotinic acetylcholine receptor were investigated by attenuated total reflection (ATR) FTIR spectroscopy and by surface plasmon resonance (SPR). FTIR-ATR spectra reveal structural details of the transmembrane protein. Surface enhanced infrared absorption (SEIRA) provides access to structural details in the close vicinity of the surface of the ATR crystal. Information about the abundance of protein substructures as well as about the orientation of those substructures are obtained. SPR was applied in order to detect changes within the adsorbed biomembrane during admission of a neurotransmitter as acetylcholine. Switching of the ion channel was successfully observed by SPR. This result is a step forward towards highly selective biosensors and bioactuators.",

author = "Gerald Steiner and Reiner Salzer and Wolfgang Fischer and Christian Kuhne",

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doi = "10.1117/12.384946",

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T1 - Surface enhanced FTIR spectroscopy and surface plasmon resonance on biomembranes

AU - Steiner, Gerald

AU - Salzer, Reiner

AU - Fischer, Wolfgang

AU - Kuhne, Christian

PY - 2000

Y1 - 2000

N2 - Lipid bilayers containing the nicotinic acetylcholine receptor were investigated by attenuated total reflection (ATR) FTIR spectroscopy and by surface plasmon resonance (SPR). FTIR-ATR spectra reveal structural details of the transmembrane protein. Surface enhanced infrared absorption (SEIRA) provides access to structural details in the close vicinity of the surface of the ATR crystal. Information about the abundance of protein substructures as well as about the orientation of those substructures are obtained. SPR was applied in order to detect changes within the adsorbed biomembrane during admission of a neurotransmitter as acetylcholine. Switching of the ion channel was successfully observed by SPR. This result is a step forward towards highly selective biosensors and bioactuators.

AB - Lipid bilayers containing the nicotinic acetylcholine receptor were investigated by attenuated total reflection (ATR) FTIR spectroscopy and by surface plasmon resonance (SPR). FTIR-ATR spectra reveal structural details of the transmembrane protein. Surface enhanced infrared absorption (SEIRA) provides access to structural details in the close vicinity of the surface of the ATR crystal. Information about the abundance of protein substructures as well as about the orientation of those substructures are obtained. SPR was applied in order to detect changes within the adsorbed biomembrane during admission of a neurotransmitter as acetylcholine. Switching of the ion channel was successfully observed by SPR. This result is a step forward towards highly selective biosensors and bioactuators.

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DO - 10.1117/12.384946

M3 - Conference article

AN - SCOPUS:0033701167

SN - 0277-786X

VL - 3918

SP - 215

EP - 221

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Biomedical Spectroscopy: Vibrational Spectroscopy and other Novel Techniques

Y2 - 26 January 2000 through 27 January 2000

ER -

Surface enhanced FTIR spectroscopy and surface plasmon resonance on biomembranes

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