TY - JOUR
T1 - Study of the reactive excited-state dynamics of delipidated bacteriorhodopsin upon surfactant treatments
AU - Cheng, Chi Wen
AU - Lee, Yuan-Pern
AU - Chu, Li Kang
PY - 2012/6/29
Y1 - 2012/6/29
N2 - The dynamics of the reactive excited-state of bacteriorhodopsin treated with various surfactants have been investigated through the measurement of pump-probe absorption spectra. Upon excitation of bR at 570 nm in the presence of ionic surfactants CTAB and SDS, the lifetime of the reactive excited-state of the all-trans protonated Schiff base, probed at 460 nm, was observed to increase up to 20%, whereas an insignificant change was observed upon addition of neutral surfactant C6E2. Measurements of steady-state absorption spectra, fluorescence, and circular dichroism indicated that the bR suspensions retain their trimeric configuration with partial delipidation. This removal of lipids causes a structural alteration and the varied excited-state dynamics.
AB - The dynamics of the reactive excited-state of bacteriorhodopsin treated with various surfactants have been investigated through the measurement of pump-probe absorption spectra. Upon excitation of bR at 570 nm in the presence of ionic surfactants CTAB and SDS, the lifetime of the reactive excited-state of the all-trans protonated Schiff base, probed at 460 nm, was observed to increase up to 20%, whereas an insignificant change was observed upon addition of neutral surfactant C6E2. Measurements of steady-state absorption spectra, fluorescence, and circular dichroism indicated that the bR suspensions retain their trimeric configuration with partial delipidation. This removal of lipids causes a structural alteration and the varied excited-state dynamics.
UR - http://www.scopus.com/inward/record.url?scp=84862654699&partnerID=8YFLogxK
U2 - 10.1016/j.cplett.2012.05.006
DO - 10.1016/j.cplett.2012.05.006
M3 - Article
AN - SCOPUS:84862654699
SN - 0009-2614
VL - 539-540
SP - 151
EP - 156
JO - Chemical Physics Letters
JF - Chemical Physics Letters
ER -