TY - JOUR
T1 - Structural characterization of collagen using polarization-resolved second harmonic generation (SHG) microscopy
AU - Mazumder, N.
AU - Sindhoora, K. M.
AU - Kao, F. J.
N1 - Publisher Copyright:
© 2021 Published under licence by IOP Publishing Ltd.
PY - 2021/4/9
Y1 - 2021/4/9
N2 - Second harmonic generation (SHG) microscopy is an effective analytical tool for a detailed investigation of the microscopic structure of non-centrosymmetric molecules. We developed a four-channel photon-counting-based Stokes polarimeter integrated to the SHG microscope for spatial characterization of polarization effects in the SH signal. We implemented Stokes-vector-based polarization-resolved SHG imaging to perform quantitative polarimetry, with a view to applications in biomedicine, which can measure the full polarization state of the SH light. In this work, we describe the application of a Stokes-vector-based four-channel photon counting SHG microscope to determine the molecular interpretation of the SH light from collagen in normal, scar, and keloid tissue.
AB - Second harmonic generation (SHG) microscopy is an effective analytical tool for a detailed investigation of the microscopic structure of non-centrosymmetric molecules. We developed a four-channel photon-counting-based Stokes polarimeter integrated to the SHG microscope for spatial characterization of polarization effects in the SH signal. We implemented Stokes-vector-based polarization-resolved SHG imaging to perform quantitative polarimetry, with a view to applications in biomedicine, which can measure the full polarization state of the SH light. In this work, we describe the application of a Stokes-vector-based four-channel photon counting SHG microscope to determine the molecular interpretation of the SH light from collagen in normal, scar, and keloid tissue.
UR - http://www.scopus.com/inward/record.url?scp=85104248541&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1859/1/012034
DO - 10.1088/1742-6596/1859/1/012034
M3 - Conference article
AN - SCOPUS:85104248541
SN - 1742-6588
VL - 1859
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 1
M1 - 012034
T2 - 21st International Conference and School on Quantum Electronics: Laser Physics and Applications, ICSQE 2020
Y2 - 21 September 2020 through 25 September 2020
ER -