Stokes vector formalism based second harmonic generation microscopy

Jianjun Qiu; Nirmal Mazumder; Han Ruei Tsai; Chih Wei Hu; Fu Jen Kao

doi:10.1117/12.907199

Stokes vector formalism based second harmonic generation microscopy

Jianjun Qiu^*, Nirmal Mazumder, Han Ruei Tsai, Chih Wei Hu, Fu Jen Kao

^*Corresponding author for this work

Institute of Biophotonics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Scopus citations

Abstract

In this study, we have developed a four-channel Stokes vector formalism based second harmonic generation (SHG) microscopy to map and analyze SHG signal. A four-channel Stokesmeter setup is calibrated and integrated into a laser scanning microscope to measure and characterize the SH's corresponding Stokes parameters. We are demonstrating the use of SH and its Stokes parameters to visualize the birefringence and crystalline orientation of KDP and collagen. We believe the developed method can reveal unprecedented information for biomedical and biomaterial studies.

Original language	English
Title of host publication	Single Molecule Spectroscopy and Superresolution Imaging V
DOIs	https://doi.org/10.1117/12.907199
State	Published - 2012
Event	Single Molecule Spectroscopy and Superresolution Imaging V - San Francisco, CA, United States Duration: 21 Jan 2012 → 22 Jan 2012

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	8228
ISSN (Print)	1605-7422

Conference

Conference	Single Molecule Spectroscopy and Superresolution Imaging V
Country/Territory	United States
City	San Francisco, CA
Period	21/01/12 → 22/01/12

Keywords

KDP
Stokes vector
collagen
polarization
second harmonic generation

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10.1117/12.907199

Cite this

@inproceedings{7d3cd98f03d84f538830125f916d78bb,

title = "Stokes vector formalism based second harmonic generation microscopy",

abstract = "In this study, we have developed a four-channel Stokes vector formalism based second harmonic generation (SHG) microscopy to map and analyze SHG signal. A four-channel Stokesmeter setup is calibrated and integrated into a laser scanning microscope to measure and characterize the SH's corresponding Stokes parameters. We are demonstrating the use of SH and its Stokes parameters to visualize the birefringence and crystalline orientation of KDP and collagen. We believe the developed method can reveal unprecedented information for biomedical and biomaterial studies.",

keywords = "KDP, Stokes vector, collagen, polarization, second harmonic generation",

author = "Jianjun Qiu and Nirmal Mazumder and Tsai, {Han Ruei} and Hu, {Chih Wei} and Kao, {Fu Jen}",

year = "2012",

doi = "10.1117/12.907199",

language = "English",

isbn = "9780819488718",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

booktitle = "Single Molecule Spectroscopy and Superresolution Imaging V",

note = "Single Molecule Spectroscopy and Superresolution Imaging V ; Conference date: 21-01-2012 Through 22-01-2012",

}

Qiu, J, Mazumder, N, Tsai, HR, Hu, CW & Kao, FJ 2012, Stokes vector formalism based second harmonic generation microscopy. in Single Molecule Spectroscopy and Superresolution Imaging V., 82280C, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8228, Single Molecule Spectroscopy and Superresolution Imaging V, San Francisco, CA, United States, 21/01/12. https://doi.org/10.1117/12.907199

Stokes vector formalism based second harmonic generation microscopy. / Qiu, Jianjun; Mazumder, Nirmal; Tsai, Han Ruei et al.
Single Molecule Spectroscopy and Superresolution Imaging V. 2012. 82280C (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8228).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Stokes vector formalism based second harmonic generation microscopy

AU - Qiu, Jianjun

AU - Mazumder, Nirmal

AU - Tsai, Han Ruei

AU - Hu, Chih Wei

AU - Kao, Fu Jen

PY - 2012

Y1 - 2012

N2 - In this study, we have developed a four-channel Stokes vector formalism based second harmonic generation (SHG) microscopy to map and analyze SHG signal. A four-channel Stokesmeter setup is calibrated and integrated into a laser scanning microscope to measure and characterize the SH's corresponding Stokes parameters. We are demonstrating the use of SH and its Stokes parameters to visualize the birefringence and crystalline orientation of KDP and collagen. We believe the developed method can reveal unprecedented information for biomedical and biomaterial studies.

AB - In this study, we have developed a four-channel Stokes vector formalism based second harmonic generation (SHG) microscopy to map and analyze SHG signal. A four-channel Stokesmeter setup is calibrated and integrated into a laser scanning microscope to measure and characterize the SH's corresponding Stokes parameters. We are demonstrating the use of SH and its Stokes parameters to visualize the birefringence and crystalline orientation of KDP and collagen. We believe the developed method can reveal unprecedented information for biomedical and biomaterial studies.

KW - KDP

KW - Stokes vector

KW - collagen

KW - polarization

KW - second harmonic generation

UR - http://www.scopus.com/inward/record.url?scp=84857594622&partnerID=8YFLogxK

U2 - 10.1117/12.907199

DO - 10.1117/12.907199

M3 - Conference contribution

AN - SCOPUS:84857594622

SN - 9780819488718

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Single Molecule Spectroscopy and Superresolution Imaging V

T2 - Single Molecule Spectroscopy and Superresolution Imaging V

Y2 - 21 January 2012 through 22 January 2012

ER -

Stokes vector formalism based second harmonic generation microscopy

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