Stimulation of proliferation and migration of mouse macrophages by type B CpG-ODNs is F-spondin and IL-1Ra dependent

Tai An Chen; Chiao Chun Liao; Yung Chih Cheng; Yen Po Chen; Yi Fan Hsu; Chi Ming Liang; Shu Mei Liang

doi:10.1371/journal.pone.0128926

Stimulation of proliferation and migration of mouse macrophages by type B CpG-ODNs is F-spondin and IL-1Ra dependent

Tai An Chen, Chiao Chun Liao, Yung Chih Cheng, Yen Po Chen, Yi Fan Hsu, Chi Ming Liang, Shu Mei Liang

Department of Tropical Medicine

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Macrophage proliferation and migration are important for many facets of immune response. Here we showed that stimulation of macrophages with type B CpG oligodeoxynucleotides (CpG-B ODNs) such as CpG-ODN 1668 increased the production of anti-inflammatory cytokine interleukin 1 receptor antagonist (IL-1Ra) in a TLR9- and MyD88-dependent manner. The CpG-B ODNs-induced IL-1Ra increased macrophage migration and promoted macrophage proliferation by down-regulating the expression of a cell cycle negative regulator, p27 to increase cell population in the S phase. The induction of IL-1Ra by CpG-B ODNs was F-spondin dependent. Knockdown of F-spondin and IL-1Ra decreased CpG-B ODNs-induced macrophage migration whereas overexpression of IL-1Ra increased migration of those cells. These findings demonstrated novel roles for F-spondin and IL-1Ra in CpG-B ODNs-mediated cell proliferation and migration of macrophages.

Original language	English
Article number	e0128926
Journal	PLoS ONE
Volume	10
Issue number	6
DOIs	https://doi.org/10.1371/journal.pone.0128926
State	Published - 4 Jun 2015

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title = "Stimulation of proliferation and migration of mouse macrophages by type B CpG-ODNs is F-spondin and IL-1Ra dependent",

abstract = "Macrophage proliferation and migration are important for many facets of immune response. Here we showed that stimulation of macrophages with type B CpG oligodeoxynucleotides (CpG-B ODNs) such as CpG-ODN 1668 increased the production of anti-inflammatory cytokine interleukin 1 receptor antagonist (IL-1Ra) in a TLR9- and MyD88-dependent manner. The CpG-B ODNs-induced IL-1Ra increased macrophage migration and promoted macrophage proliferation by down-regulating the expression of a cell cycle negative regulator, p27 to increase cell population in the S phase. The induction of IL-1Ra by CpG-B ODNs was F-spondin dependent. Knockdown of F-spondin and IL-1Ra decreased CpG-B ODNs-induced macrophage migration whereas overexpression of IL-1Ra increased migration of those cells. These findings demonstrated novel roles for F-spondin and IL-1Ra in CpG-B ODNs-mediated cell proliferation and migration of macrophages.",

author = "Chen, {Tai An} and Liao, {Chiao Chun} and Cheng, {Yung Chih} and Chen, {Yen Po} and Hsu, {Yi Fan} and Liang, {Chi Ming} and Liang, {Shu Mei}",

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AU - Chen, Tai An

AU - Liao, Chiao Chun

AU - Cheng, Yung Chih

AU - Chen, Yen Po

AU - Hsu, Yi Fan

AU - Liang, Chi Ming

AU - Liang, Shu Mei

N1 - Publisher Copyright: © 2015 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2015/6/4

Y1 - 2015/6/4

N2 - Macrophage proliferation and migration are important for many facets of immune response. Here we showed that stimulation of macrophages with type B CpG oligodeoxynucleotides (CpG-B ODNs) such as CpG-ODN 1668 increased the production of anti-inflammatory cytokine interleukin 1 receptor antagonist (IL-1Ra) in a TLR9- and MyD88-dependent manner. The CpG-B ODNs-induced IL-1Ra increased macrophage migration and promoted macrophage proliferation by down-regulating the expression of a cell cycle negative regulator, p27 to increase cell population in the S phase. The induction of IL-1Ra by CpG-B ODNs was F-spondin dependent. Knockdown of F-spondin and IL-1Ra decreased CpG-B ODNs-induced macrophage migration whereas overexpression of IL-1Ra increased migration of those cells. These findings demonstrated novel roles for F-spondin and IL-1Ra in CpG-B ODNs-mediated cell proliferation and migration of macrophages.

AB - Macrophage proliferation and migration are important for many facets of immune response. Here we showed that stimulation of macrophages with type B CpG oligodeoxynucleotides (CpG-B ODNs) such as CpG-ODN 1668 increased the production of anti-inflammatory cytokine interleukin 1 receptor antagonist (IL-1Ra) in a TLR9- and MyD88-dependent manner. The CpG-B ODNs-induced IL-1Ra increased macrophage migration and promoted macrophage proliferation by down-regulating the expression of a cell cycle negative regulator, p27 to increase cell population in the S phase. The induction of IL-1Ra by CpG-B ODNs was F-spondin dependent. Knockdown of F-spondin and IL-1Ra decreased CpG-B ODNs-induced macrophage migration whereas overexpression of IL-1Ra increased migration of those cells. These findings demonstrated novel roles for F-spondin and IL-1Ra in CpG-B ODNs-mediated cell proliferation and migration of macrophages.

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Stimulation of proliferation and migration of mouse macrophages by type B CpG-ODNs is F-spondin and IL-1Ra dependent

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