Stimulated emission and spontaneous loss pump-probe microscopy for background removal

Subir Das*, Bo Wei Ho, Fu Jen Kao

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

In this work, we have established a double modulation lock-in detection technique using two semiconductor laser diodes in stimulated emission based pump-probe microscopy. By modulating the pump and probe beams at two different frequencies, f1 and f2, the signal is then recovered with the sum frequency, (f1+ f2), so as to minimize the leak-through noise due to the spontaneous emission caused by the pump beam. In this way, the DC background that is often attributed to the stimulated emission is effectively removed. Our technique has implemented in ATTO647N fluorescent dye which is applicable for many biological applications.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XVIII
EditorsAmmasi Periasamy, Xiaoliang S. Xie, Xiaoliang S. Xie, Karsten Konig, Peter T. C. So
PublisherSPIE
ISBN (Electronic)9781510614819
DOIs
StatePublished - 2018
EventMultiphoton Microscopy in the Biomedical Sciences XVIII 2018 - San Francisco, United States
Duration: 28 Jan 201830 Jan 2018

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10498
ISSN (Print)1605-7422

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XVIII 2018
Country/TerritoryUnited States
CitySan Francisco
Period28/01/1830/01/18

Keywords

  • ATTO647N
  • Lock-in detection
  • Pump-probe microscopy
  • Spontaneous loss
  • Stimulated emission

Fingerprint

Dive into the research topics of 'Stimulated emission and spontaneous loss pump-probe microscopy for background removal'. Together they form a unique fingerprint.

Cite this