@inproceedings{8e2108466cd042388bd7702a726c42ce,
title = "Stimulated emission and spontaneous loss pump-probe microscopy for background removal",
abstract = "In this work, we have established a double modulation lock-in detection technique using two semiconductor laser diodes in stimulated emission based pump-probe microscopy. By modulating the pump and probe beams at two different frequencies, f1 and f2, the signal is then recovered with the sum frequency, (f1+ f2), so as to minimize the leak-through noise due to the spontaneous emission caused by the pump beam. In this way, the DC background that is often attributed to the stimulated emission is effectively removed. Our technique has implemented in ATTO647N fluorescent dye which is applicable for many biological applications.",
keywords = "ATTO647N, Lock-in detection, Pump-probe microscopy, Spontaneous loss, Stimulated emission",
author = "Subir Das and Ho, {Bo Wei} and Kao, {Fu Jen}",
note = "Publisher Copyright: {\textcopyright} 2018 SPIE.; Multiphoton Microscopy in the Biomedical Sciences XVIII 2018 ; Conference date: 28-01-2018 Through 30-01-2018",
year = "2018",
doi = "10.1117/12.2284396",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Ammasi Periasamy and Xie, {Xiaoliang S.} and Xie, {Xiaoliang S.} and Karsten Konig and So, {Peter T. C.}",
booktitle = "Multiphoton Microscopy in the Biomedical Sciences XVIII",
address = "美國",
}