Sterically polymer-based liposomal complexes with dual-shell structure for enhancing the siRNA delivery

Shuian Yin Lin; Wei Yu Zhao; Hsieh Chih Tsai; Wei Hsin Hsu; Chun Liang Lo; Ging Ho Hsiue

doi:10.1021/bm201746t

Sterically polymer-based liposomal complexes with dual-shell structure for enhancing the siRNA delivery

Shuian Yin Lin, Wei Yu Zhao, Hsieh Chih Tsai, Wei Hsin Hsu, Chun Liang Lo^*, Ging Ho Hsiue

^*Corresponding author for this work

Department of Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The sterically polymer-based liposomal complexes (SPLexes) were formed by cationic polymeric liposomes and pH-sensitive diblock copolymer were studied for their capabilities in improving the stability with high efficiency of siRNA delivery. The SPLexes were formed a dual-shelled structure and uniform size distribution. The PEGylated outer shell could mitigate the phagocytosis and reduce the cytotoxicity. Moreover, the folated SPLexes improved 42.9× accumulation in vitro and 1.7× tumor uptake in vivo in contrast with nonfolated SPLexes. The protonated copolymer at low pH would improve the siRNA released into cytoplasm following SPLexes fusion with the endo/lysosome membrane and inhibited the protein expression to 75.6 ± 4.5% efficiently. Results of this study significantly contribute to efforts to develop lipoplexes based siRNA delivery systems.

Original language	English
Pages (from-to)	664-675
Number of pages	12
Journal	Biomacromolecules
Volume	13
Issue number	3
DOIs	https://doi.org/10.1021/bm201746t
State	Published - 12 Mar 2012

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title = "Sterically polymer-based liposomal complexes with dual-shell structure for enhancing the siRNA delivery",

abstract = "The sterically polymer-based liposomal complexes (SPLexes) were formed by cationic polymeric liposomes and pH-sensitive diblock copolymer were studied for their capabilities in improving the stability with high efficiency of siRNA delivery. The SPLexes were formed a dual-shelled structure and uniform size distribution. The PEGylated outer shell could mitigate the phagocytosis and reduce the cytotoxicity. Moreover, the folated SPLexes improved 42.9× accumulation in vitro and 1.7× tumor uptake in vivo in contrast with nonfolated SPLexes. The protonated copolymer at low pH would improve the siRNA released into cytoplasm following SPLexes fusion with the endo/lysosome membrane and inhibited the protein expression to 75.6 ± 4.5% efficiently. Results of this study significantly contribute to efforts to develop lipoplexes based siRNA delivery systems.",

author = "Lin, {Shuian Yin} and Zhao, {Wei Yu} and Tsai, {Hsieh Chih} and Hsu, {Wei Hsin} and Lo, {Chun Liang} and Hsiue, {Ging Ho}",

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AU - Lin, Shuian Yin

AU - Zhao, Wei Yu

AU - Tsai, Hsieh Chih

AU - Hsu, Wei Hsin

AU - Lo, Chun Liang

AU - Hsiue, Ging Ho

PY - 2012/3/12

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Sterically polymer-based liposomal complexes with dual-shell structure for enhancing the siRNA delivery

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