Stage-Specific Differences in the Requirements for Germline Stem Cell Maintenance in the Drosophila Ovary

Halyna R. Shcherbata; Ellen J. Ward; Karin A. Fischer; Jenn Yah Yu; Steven H. Reynolds; Chun Hong Chen; Peizhang Xu; Bruce A. Hay; Hannele Ruohola-Baker

doi:10.1016/j.stem.2007.11.007

Stage-Specific Differences in the Requirements for Germline Stem Cell Maintenance in the Drosophila Ovary

Halyna R. Shcherbata, Ellen J. Ward, Karin A. Fischer, Jenn Yah Yu, Steven H. Reynolds, Chun Hong Chen, Peizhang Xu, Bruce A. Hay, Hannele Ruohola-Baker^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

In this study, we uncover a role for microRNAs (miRNAs) in Drosophila germline stem cell (GSC) maintenance. Disruption of Dicer-1 function in GSCs during adult life results in GSC loss. Surprisingly, however, loss of Dicer-1 during development does not result in a GSC maintenance defect, although a defect is seen if both Dicer-1 and Dicer-2 function are disrupted. Loss of the bantam miRNA mimics the Dicer-1 maintenance defect when induced in adult GSCs, suggesting that bantam plays a key role in GSC self-renewal. Mad, a component of the TGF-β pathway, behaves similarly to Dicer-1: adult GSC maintenance requires Mad if it is lost during adult life, but not if it is lost during pupal development. Overall, these results show stage-specific differential sensitivity of GSC maintenance to certain perturbations and suggest that there may be a Dicer-2-dependent GSC maintenance mechanism during development that is lost in later life.

Original language	English
Pages (from-to)	698-709
Number of pages	12
Journal	Cell Stem Cell
Volume	1
Issue number	6
DOIs	https://doi.org/10.1016/j.stem.2007.11.007
State	Published - 13 Dec 2007

Keywords

STEMCELL

Access to Document

10.1016/j.stem.2007.11.007

Cite this

@article{68ad30644aa148ecb123a8b4d8bd4590,

title = "Stage-Specific Differences in the Requirements for Germline Stem Cell Maintenance in the Drosophila Ovary",

abstract = "In this study, we uncover a role for microRNAs (miRNAs) in Drosophila germline stem cell (GSC) maintenance. Disruption of Dicer-1 function in GSCs during adult life results in GSC loss. Surprisingly, however, loss of Dicer-1 during development does not result in a GSC maintenance defect, although a defect is seen if both Dicer-1 and Dicer-2 function are disrupted. Loss of the bantam miRNA mimics the Dicer-1 maintenance defect when induced in adult GSCs, suggesting that bantam plays a key role in GSC self-renewal. Mad, a component of the TGF-β pathway, behaves similarly to Dicer-1: adult GSC maintenance requires Mad if it is lost during adult life, but not if it is lost during pupal development. Overall, these results show stage-specific differential sensitivity of GSC maintenance to certain perturbations and suggest that there may be a Dicer-2-dependent GSC maintenance mechanism during development that is lost in later life.",

keywords = "STEMCELL",

author = "Shcherbata, {Halyna R.} and Ward, {Ellen J.} and Fischer, {Karin A.} and Yu, {Jenn Yah} and Reynolds, {Steven H.} and Chen, {Chun Hong} and Peizhang Xu and Hay, {Bruce A.} and Hannele Ruohola-Baker",

note = "Funding Information: We thank Drs. A. Spradling, D. Pan, B. Calvi, S. Cohen, T.T. Su, T. Orr-Weaver, T. Xie, J. Tamkun, and R. Carthew for suggestions, flies, and reagents, Christian Nelson, Louise von Stechow, and Andriy Yatsenko for help with experiments, and Dr. Bradford Stadler for help with QPCR analysis. This work was supported by AHA fellowships for H.R.S. and S.H.R. and MOD and NIH grants and the Tietze Fellowship for H.R-B. ",

year = "2007",

month = dec,

day = "13",

doi = "10.1016/j.stem.2007.11.007",

language = "English",

volume = "1",

pages = "698--709",

journal = "Cell Stem Cell",

issn = "1934-5909",

publisher = "Cell Press",

number = "6",

}

TY - JOUR

T1 - Stage-Specific Differences in the Requirements for Germline Stem Cell Maintenance in the Drosophila Ovary

AU - Shcherbata, Halyna R.

AU - Ward, Ellen J.

AU - Fischer, Karin A.

AU - Yu, Jenn Yah

AU - Reynolds, Steven H.

AU - Chen, Chun Hong

AU - Xu, Peizhang

AU - Hay, Bruce A.

AU - Ruohola-Baker, Hannele

N1 - Funding Information: We thank Drs. A. Spradling, D. Pan, B. Calvi, S. Cohen, T.T. Su, T. Orr-Weaver, T. Xie, J. Tamkun, and R. Carthew for suggestions, flies, and reagents, Christian Nelson, Louise von Stechow, and Andriy Yatsenko for help with experiments, and Dr. Bradford Stadler for help with QPCR analysis. This work was supported by AHA fellowships for H.R.S. and S.H.R. and MOD and NIH grants and the Tietze Fellowship for H.R-B.

PY - 2007/12/13

Y1 - 2007/12/13

N2 - In this study, we uncover a role for microRNAs (miRNAs) in Drosophila germline stem cell (GSC) maintenance. Disruption of Dicer-1 function in GSCs during adult life results in GSC loss. Surprisingly, however, loss of Dicer-1 during development does not result in a GSC maintenance defect, although a defect is seen if both Dicer-1 and Dicer-2 function are disrupted. Loss of the bantam miRNA mimics the Dicer-1 maintenance defect when induced in adult GSCs, suggesting that bantam plays a key role in GSC self-renewal. Mad, a component of the TGF-β pathway, behaves similarly to Dicer-1: adult GSC maintenance requires Mad if it is lost during adult life, but not if it is lost during pupal development. Overall, these results show stage-specific differential sensitivity of GSC maintenance to certain perturbations and suggest that there may be a Dicer-2-dependent GSC maintenance mechanism during development that is lost in later life.

AB - In this study, we uncover a role for microRNAs (miRNAs) in Drosophila germline stem cell (GSC) maintenance. Disruption of Dicer-1 function in GSCs during adult life results in GSC loss. Surprisingly, however, loss of Dicer-1 during development does not result in a GSC maintenance defect, although a defect is seen if both Dicer-1 and Dicer-2 function are disrupted. Loss of the bantam miRNA mimics the Dicer-1 maintenance defect when induced in adult GSCs, suggesting that bantam plays a key role in GSC self-renewal. Mad, a component of the TGF-β pathway, behaves similarly to Dicer-1: adult GSC maintenance requires Mad if it is lost during adult life, but not if it is lost during pupal development. Overall, these results show stage-specific differential sensitivity of GSC maintenance to certain perturbations and suggest that there may be a Dicer-2-dependent GSC maintenance mechanism during development that is lost in later life.

KW - STEMCELL

UR - http://www.scopus.com/inward/record.url?scp=36749004987&partnerID=8YFLogxK

U2 - 10.1016/j.stem.2007.11.007

DO - 10.1016/j.stem.2007.11.007

M3 - Article

C2 - 18213359

AN - SCOPUS:36749004987

SN - 1934-5909

VL - 1

SP - 698

EP - 709

JO - Cell Stem Cell

JF - Cell Stem Cell

IS - 6

ER -

Stage-Specific Differences in the Requirements for Germline Stem Cell Maintenance in the Drosophila Ovary

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this