Specific two-photon fluorescent probe for cysteine detection in vivo

Yu Xu Tu; Natarajan Vijay; Han Xiang Ko; Yuan Pin Lo; Sivan Velmathi; Shu Pao Wu

doi:10.1016/j.saa.2021.120521

Specific two-photon fluorescent probe for cysteine detection in vivo

Yu Xu Tu, Natarajan Vijay, Han Xiang Ko, Yuan Pin Lo, Sivan Velmathi^*, Shu Pao Wu

^*Corresponding author for this work

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Cysteine (Cys), an essential amino acid, plays several crucial functions in numerous biological processes. Notably, the detection of Cys is critical to disease diagnosis. Fluorescent probes that can quickly detect Cys will help to study the mechanism of certain diseases. Herein, a new fluorescent probe, ANP, which is based on 6-acetyl-N-methyl-2-naphthyl amine, has been developed for Cys detection over Hcy and GSH in vivo. The addition of thiol on α,β-unsaturated ketone promotes 87-fold fluorescence turn-on response with a 65 nM limit of detection. The high two-photon efficiency of the probe ANP (cross-section = 22.3) makes it a suitable probe for evaluating Cys in living cells without background fluorescence interference. Its application was extended to monitor the Cys distribution in live cells and tissues.

Original language	English
Article number	120521
Journal	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume	267
DOIs	https://doi.org/10.1016/j.saa.2021.120521
State	Published - 15 Feb 2022

Keywords

Bioimaging
Cysteine
Fluorescent probe
Two-Photon

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10.1016/j.saa.2021.120521

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title = "Specific two-photon fluorescent probe for cysteine detection in vivo",

abstract = "Cysteine (Cys), an essential amino acid, plays several crucial functions in numerous biological processes. Notably, the detection of Cys is critical to disease diagnosis. Fluorescent probes that can quickly detect Cys will help to study the mechanism of certain diseases. Herein, a new fluorescent probe, ANP, which is based on 6-acetyl-N-methyl-2-naphthyl amine, has been developed for Cys detection over Hcy and GSH in vivo. The addition of thiol on α,β-unsaturated ketone promotes 87-fold fluorescence turn-on response with a 65 nM limit of detection. The high two-photon efficiency of the probe ANP (cross-section = 22.3) makes it a suitable probe for evaluating Cys in living cells without background fluorescence interference. Its application was extended to monitor the Cys distribution in live cells and tissues.",

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T1 - Specific two-photon fluorescent probe for cysteine detection in vivo

AU - Tu, Yu Xu

AU - Vijay, Natarajan

AU - Ko, Han Xiang

AU - Lo, Yuan Pin

AU - Velmathi, Sivan

AU - Wu, Shu Pao

PY - 2022/2/15

Y1 - 2022/2/15

N2 - Cysteine (Cys), an essential amino acid, plays several crucial functions in numerous biological processes. Notably, the detection of Cys is critical to disease diagnosis. Fluorescent probes that can quickly detect Cys will help to study the mechanism of certain diseases. Herein, a new fluorescent probe, ANP, which is based on 6-acetyl-N-methyl-2-naphthyl amine, has been developed for Cys detection over Hcy and GSH in vivo. The addition of thiol on α,β-unsaturated ketone promotes 87-fold fluorescence turn-on response with a 65 nM limit of detection. The high two-photon efficiency of the probe ANP (cross-section = 22.3) makes it a suitable probe for evaluating Cys in living cells without background fluorescence interference. Its application was extended to monitor the Cys distribution in live cells and tissues.

AB - Cysteine (Cys), an essential amino acid, plays several crucial functions in numerous biological processes. Notably, the detection of Cys is critical to disease diagnosis. Fluorescent probes that can quickly detect Cys will help to study the mechanism of certain diseases. Herein, a new fluorescent probe, ANP, which is based on 6-acetyl-N-methyl-2-naphthyl amine, has been developed for Cys detection over Hcy and GSH in vivo. The addition of thiol on α,β-unsaturated ketone promotes 87-fold fluorescence turn-on response with a 65 nM limit of detection. The high two-photon efficiency of the probe ANP (cross-section = 22.3) makes it a suitable probe for evaluating Cys in living cells without background fluorescence interference. Its application was extended to monitor the Cys distribution in live cells and tissues.

KW - Bioimaging

KW - Cysteine

KW - Fluorescent probe

KW - Two-Photon

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U2 - 10.1016/j.saa.2021.120521

DO - 10.1016/j.saa.2021.120521

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SN - 1386-1425

VL - 267

JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

M1 - 120521

ER -

Specific two-photon fluorescent probe for cysteine detection in vivo

Abstract

Keywords

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