Spatially reinforced Au nano-cavities as a reaction nano-reservoir for trace analysis of DNA hybridization

Chih Kai Yao, Jiunn Der Liao*, Chih Heng Lin, Yuh-Shyong Yang, Sheng Hong Yu, Jung Wei Yang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Spatially reinforced Au nano-cavities (SR-nAu) with reduced tip-to-tip displacement create strongly localized surface plasmon resonance by Raman scattering. Within SR-nAu, the nano-cavity structure with high aspect ratio was optimized as the nano-reservoir for the distinction of DNA hybridization responses from specific DNA sequences of Avian influenza virus. In the nano-reservoir, the suspended or immobilized probe, its complementary and noncomplementary target sequences were characterized at very low concentration through enhanced Raman spectra. The presence of Raman shift at 738 cm -1, the characteristic breathing mode of thymine, and the change of relative peaks intensity at 1603 and 1554 cm-1 before and after hybridization were particularly taken as the major indications to validate the match or mismatch state of DNA sequences. Through a labeling-free means, and from the evidence provided by specific complementary and noncomplementary DNA sequences, the recognition capability of nano-reservoir can be down to subnano-molar concentration (10-10 M).

Original languageEnglish
Pages (from-to)219-226
Number of pages8
JournalSensors and Actuators, B: Chemical
StatePublished - Feb 2014


  • DNA hybridization
  • Labeling-free
  • Nano-reservoir
  • Raman shift
  • Spatially reinforced Au nano-cavities


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