Single-virus tracking approach to reveal the interaction of Dengue virus with autophagy during the early stage of infection.

Li Wei Chu*, Yi Lung Huang, Jin Hui Lee, Long Ying Huang, Wei Jun Chen, Ya Hsuan Lin, Jyun Yu Chen, Rui Xiang, Chau Hwang Lee, Yueh Hsin Ping

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Dengue virus (DENV) is one of the major infectious pathogens worldwide. DENV infection is a highly dynamic process. Currently, no antiviral drug is available for treating DENV-induced diseases since little is known regarding how the virus interacts with host cells during infection. Advanced molecular imaging technologies are powerful tools to understand the dynamics of intracellular interactions and molecular trafficking. This study exploited a single-virus particle tracking technology to address whether DENV interacts with autophagy machinery during the early stage of infection. Using confocal microscopy and three-dimensional image analysis, we showed that DENV triggered the formation of green fluorescence protein-fused microtubule-associated protein 1A/1B-light chain 3 (GFP-LC3) puncta, and DENV-induced autophagosomes engulfed DENV particles within 15-min postinfection. Moreover, single-virus particle tracking revealed that both DENV particles and autophagosomes traveled together during the viral infection. Finally, in the presence of autophagy suppressor 3-methyladenine, the replication of DENV was inhibited and the location of DENV particles spread in cytoplasma. In contrast, the numbers of newly synthesized DENV were elevated and the co-localization of DENV particles and autophagosomes was detected while the cells were treated with autophagy inducer rapamycin. Taken together, we propose that DENV particles interact with autophagosomes at the early stage of viral infection, which promotes the replication of DENV.

Original languageEnglish
Pages (from-to)11018
Number of pages1
JournalJournal of Biomedical Optics
Volume19
Issue number1
DOIs
StatePublished - Jan 2014

Fingerprint

Dive into the research topics of 'Single-virus tracking approach to reveal the interaction of Dengue virus with autophagy during the early stage of infection.'. Together they form a unique fingerprint.

Cite this