Abstract
Single cell manipulation is one of the key methods in single cell technologies. A cell can be positioned to a designation at a specified velocity for performing tasks, such as cell evaluation and cell sorting. This chapter is started with the state-of-the-art technologies of single cell manipulation, which include manipulations with both micro-scale and macro-scale actuators. Manipulation with a macro-scale actuator has advantages of low-cost and easy-access, but is challenging to control due to scale difference of several orders. Therefore, the introduction will be followed by a review of single cell manipulations using a macro-scale actuator. Transfer function of the macro-to-micro manipulation is derived based on a mechanical model incorporating the deformation of microfluidic chip. The coefficients of the transfer function are determined based on experimental results. An advanced manipulation system with closed-loop control and an on-chip transmitter is presented with experimental results. Finally, applications on cell evaluation using macro-scale actuator will be discussed at the end of the chapter.
Original language | English |
---|---|
Title of host publication | Handbook of Single-Cell Technologies |
Publisher | Springer Singapore |
Pages | 137-154 |
Number of pages | 18 |
ISBN (Electronic) | 9789811089534 |
ISBN (Print) | 9789811089527 |
DOIs | |
State | Published - 1 Jan 2021 |