Simultaneous Saccharification and Fermentation of Waste Textiles for Ethanol Production

Chia Hung Kuo*, Po Ju Lin, Yu Qiong Wu, Li Yi Ye, Deng Jye Yang, Chwen Jen Shieh, Cheng Kang Lee

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Ethanol production in a simultaneous saccharification and fermentation (SSF) process using waste textiles as feedstock was studied. The dissolution pretreatment of waste textiles in ortho-phosphoric acid resulted in at least 2 fold improvement in enzymatic hydrolysis rate and reducing sugar yield. The reducing sugars obtained from dyed or discolored waste textiles by cellulase hydrolysis demonstrated no inhibitory effect on ethanol fermentation activity of Zymomonas mobilis employed in SSF. SSF with a high waste textile loading (75 g L-1) could still be operable due to the fast liquefaction of the pretreated substrate via enzymatic hydrolysis. Approximately 50 g L-1 ethanol was achieved within 24 h. In addition to 100% cotton textiles, the 40/60 polyester/cotton (T/C) blend waste textile could also be pretreated under the same condition to achieve the comparable ethanol production yield (~0.4 g EtOH g-1 glucose) from its cotton fraction in SSF.

Original languageEnglish
Pages (from-to)2866-2875
Number of pages10
Issue number2
StatePublished - May 2014


  • Ethanol
  • Phosphoric acid
  • Pretreatment
  • Simultaneous saccharification and fermentation
  • Waste textile


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