TY - JOUR
T1 - Simultaneous determination of urinary metabolites of toluene, xylene, styrene and ethyl benzene by solid-phase extraction technique and high-performance liquid chromatographic/photo diode array detection
AU - Mao, I. F.
AU - Chen, M. L.
AU - Lo, E. W.
N1 - Funding Information:
This study was supported by a grant (No. NSC83-0421-D-010-049Z) from the National Science Council, Republic of China.
PY - 1996
Y1 - 1996
N2 - To determine the level of urinary hippuric acid, methylhippuric acid, mandelic acid, and phenylglyoxylic acid, a method by solid-phase extraction (SPE) pretreatment and reversed-phase high performance liquid chromatography with photo diode array detection has been developed in this study. Six milliliter of methanol is the only solvent used in the SPE procedures. After extraction, the urine sample is injected onto a 5 μm RP18-LiChrospher 100 column (150 mm × 4 mm I.D.). The mobile phase is a mixture of acetonitrile (15 parts) and phosphate buffer (85 parts) containing tetrabutyl ammonium hydrogen sulfate (8.5 mM). The entire procedure is performed in 40 min. The method is simple, rapid and highly specific, and applicable in field study of toluene, xylene, styrene and ethyl benzene exposure.
AB - To determine the level of urinary hippuric acid, methylhippuric acid, mandelic acid, and phenylglyoxylic acid, a method by solid-phase extraction (SPE) pretreatment and reversed-phase high performance liquid chromatography with photo diode array detection has been developed in this study. Six milliliter of methanol is the only solvent used in the SPE procedures. After extraction, the urine sample is injected onto a 5 μm RP18-LiChrospher 100 column (150 mm × 4 mm I.D.). The mobile phase is a mixture of acetonitrile (15 parts) and phosphate buffer (85 parts) containing tetrabutyl ammonium hydrogen sulfate (8.5 mM). The entire procedure is performed in 40 min. The method is simple, rapid and highly specific, and applicable in field study of toluene, xylene, styrene and ethyl benzene exposure.
KW - Hippuric acid
KW - Mandelic acid
KW - Methylhippuric acid
KW - Phenylglyoxylic acid
KW - Photo diode array detection
KW - Solid phase extraction
UR - http://www.scopus.com/inward/record.url?scp=0030479361&partnerID=8YFLogxK
U2 - 10.1080/03067319608028330
DO - 10.1080/03067319608028330
M3 - Article
AN - SCOPUS:0030479361
SN - 0306-7319
VL - 64
SP - 1
EP - 9
JO - International Journal of Environmental Analytical Chemistry
JF - International Journal of Environmental Analytical Chemistry
IS - 1
ER -
