SERS quantitative urine creatinine measurement of human subject

T. L. Wang, H. K. Chiang*, H. Lu, Y. Hung

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

4 Scopus citations

Abstract

SERS method for biomolecular analysis has several potentials and advantages over traditional biochemical approaches, including less specimen contact, non-destructive to specimen, and multiple components analysis. Urine is an easily available body fluid for monitoring the metabolites and renal function of human body. We developed surface-enhanced Raman scattering (SERS) technique using 50nm size gold colloidal particles for quantitative human urine creatinine measurements. This paper shows that SERS shifts of creatinine (104mg/dl) in artificial urine is from 1400cm-1 to 1500cm-1 which was analyzed for quantitative creatinine measurement. Ten human urine samples were obtained from ten healthy persons and analyzed by the SERS technique. Partial least square cross-validation (PLSCV) method was utilized to obtain the estimated creatinine concentration in clinically relevant (55.9mg/dl to 208mg/dl) concentration range. The root-mean square error of cross validation (RMSECV) is 26.1mg/dl. This research demonstrates the feasibility of using SERS for human subject urine creatinine detection, and establishes the SERS platform technique for bodily fluids measurement.

Original languageEnglish
Article number03
Pages (from-to)17-24
Number of pages8
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5703
DOIs
StatePublished - 2005
EventPlasmonics in Biology and Medicine II - San Jose, CA, United States
Duration: 24 Jan 200525 Jan 2005

Keywords

  • Creatinine
  • Nanoparticles
  • Quantitative analysis
  • SERS
  • Urine

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