Sensitive Detection of Oral Leukoplakia: Analyzing P90 Biomarkers in Saliva and Tissue

Hsiao Hsuan Wan, Haochen Zhu, Chao Ching Chiang, Jian Sian Li, Fan Ren, Cheng Tse Tsai, Yu Te Liao, Dan Neal, Joseph Katz, Josephine F. Esquivel-Upshaw*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Oral cancer represents a significant global public health challenge, contributing substantially to the incidence and mortality of cancer. Despite established risk factors such as tobacco use and alcohol consumption, early detection remains crucial for effective treatment. This study introduces a novel approach using a transistor-based biosensor system for detecting the P90 (CIP2A) protein. We tested the presence of CIP2A in human leukoplakia samples, which can undergo malignant conversion into aggressive oral squamous cell carcinoma. The method used commercially available glucose test strips functionalized with P90 antibodies, providing high sensitivity and a low limit of detection which was five orders lower than that of commercial ELISA kits. A specially designed printed circuit board (PCB) facilitated accurate measurements, and the device’s performance was optimized through characteristic tests. Human sample testing validated the biosensor’s effectiveness in distinguishing samples after cell lysis. This study contributes to advancing accurate and cost-effective diagnostic approaches for oral pre-cancer and cancer tissues.

Original languageEnglish
Article number281
JournalBiosensors
Volume14
Issue number6
DOIs
StatePublished - Jun 2024

Keywords

  • MOSFET
  • P90
  • high sensitivity
  • oral cancer detection
  • oral leukoplakia
  • salivary biomarkers
  • tissue

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