Second harmonic generation of biotin and biotin ester microcrystals trapped in optical tweezers with a mode-locked Ti:Sapphire laser

E. V. Perevedentseva; A. V. Karmenyan; F. J. Kao; Arthur Chiou

Second harmonic generation of biotin and biotin ester microcrystals trapped in optical tweezers with a mode-locked Ti:Sapphire laser

E. V. Perevedentseva, A. V. Karmenyan, F. J. Kao, Arthur Chiou^*

^*Corresponding author for this work

Institute of Biophotonics

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Intense second harmonic generation (SHG) was observed from microcrystals of biotin and biotin ester trapped by optical tweezers, formed with a focused near-infrared pulsed laser beam. The intensity of SHG depends strongly on the states of the microcrystals and the excitation wavelength. Microscopic scanning images of biotin and biotin ester were obtained in high contrast with SHG. Simultaneous trapping and excitation of SHG and two-photon autofluorescence of biotin and biotin ester microcrystals allow us to investigate their structure and optical properties. These optically trapped particles (of submicron size) are useful as nonintrusive microscopic probes for high-resolution studies.

Original language	English
Pages (from-to)	I78-I82
Number of pages	5
Journal	Scanning
Volume	26
Issue number	5 SUPPL.
State	Published - Sep 2004

Keywords

Biotin
Biotin ester
Optical trapping
Second harmonic generation
Two-photon fluorescence

Cite this

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title = "Second harmonic generation of biotin and biotin ester microcrystals trapped in optical tweezers with a mode-locked Ti:Sapphire laser",

abstract = "Intense second harmonic generation (SHG) was observed from microcrystals of biotin and biotin ester trapped by optical tweezers, formed with a focused near-infrared pulsed laser beam. The intensity of SHG depends strongly on the states of the microcrystals and the excitation wavelength. Microscopic scanning images of biotin and biotin ester were obtained in high contrast with SHG. Simultaneous trapping and excitation of SHG and two-photon autofluorescence of biotin and biotin ester microcrystals allow us to investigate their structure and optical properties. These optically trapped particles (of submicron size) are useful as nonintrusive microscopic probes for high-resolution studies.",

keywords = "Biotin, Biotin ester, Optical trapping, Second harmonic generation, Two-photon fluorescence",

author = "Perevedentseva, {E. V.} and Karmenyan, {A. V.} and Kao, {F. J.} and Arthur Chiou",

year = "2004",

month = sep,

language = "English",

volume = "26",

pages = "I78--I82",

journal = "Scanning",

issn = "0161-0457",

publisher = "John Wiley and Sons Inc.",

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TY - JOUR

T1 - Second harmonic generation of biotin and biotin ester microcrystals trapped in optical tweezers with a mode-locked Ti:Sapphire laser

AU - Perevedentseva, E. V.

AU - Karmenyan, A. V.

AU - Kao, F. J.

AU - Chiou, Arthur

PY - 2004/9

Y1 - 2004/9

N2 - Intense second harmonic generation (SHG) was observed from microcrystals of biotin and biotin ester trapped by optical tweezers, formed with a focused near-infrared pulsed laser beam. The intensity of SHG depends strongly on the states of the microcrystals and the excitation wavelength. Microscopic scanning images of biotin and biotin ester were obtained in high contrast with SHG. Simultaneous trapping and excitation of SHG and two-photon autofluorescence of biotin and biotin ester microcrystals allow us to investigate their structure and optical properties. These optically trapped particles (of submicron size) are useful as nonintrusive microscopic probes for high-resolution studies.

AB - Intense second harmonic generation (SHG) was observed from microcrystals of biotin and biotin ester trapped by optical tweezers, formed with a focused near-infrared pulsed laser beam. The intensity of SHG depends strongly on the states of the microcrystals and the excitation wavelength. Microscopic scanning images of biotin and biotin ester were obtained in high contrast with SHG. Simultaneous trapping and excitation of SHG and two-photon autofluorescence of biotin and biotin ester microcrystals allow us to investigate their structure and optical properties. These optically trapped particles (of submicron size) are useful as nonintrusive microscopic probes for high-resolution studies.

KW - Biotin

KW - Biotin ester

KW - Optical trapping

KW - Second harmonic generation

KW - Two-photon fluorescence

UR - http://www.scopus.com/inward/record.url?scp=6344242858&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:6344242858

SN - 0161-0457

VL - 26

SP - I78-I82

JO - Scanning

JF - Scanning

IS - 5 SUPPL.

ER -

Second harmonic generation of biotin and biotin ester microcrystals trapped in optical tweezers with a mode-locked Ti:Sapphire laser

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Keywords

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