Second-harmonic generation microscopy of tooth

Fu Jen Kao*, Yung Shun Wang, Mao Kuo Huang, Sheng Lung Huang, Ping Chin Cheng

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

7 Scopus citations


In this study, we have developed a high performance microscopic system to perform second-harmonic (SH) imaging on a tooth. The high sensitivity of the system allows acquisition rate of 300 seconds/frame with resolution at 512×512 pixels. The surface SH signal generated from the tooth is also carefully verified through micro-spectroscopy, polarization rotation, and wavelength tuning. In this way, we can ensure the authenticity of the signal. The enamel that encapsulates the dentine is known to possess highly ordered structures. The anisotropy of the structure is revealed in the microscopic SH images of the tooth sample.

Original languageEnglish
Pages (from-to)119-124
Number of pages6
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 2000
EventOptical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications - Taipei, Taiwan
Duration: 26 Jul 200028 Jul 2000


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